Journal article
Trypanosoma cruzi exhibits inter- and intra-strain heterogeneity in molecular karyotype and chromosomal gene location
Molecular and biochemical parasitology, Vol.22(2), pp.115-123
1987
DOI: 10.1016/0166-6851(87)90041-7
PMID: 3553931
Abstract
Molecular karyotypes of 6 strains and 6 clones of
Trypanosoma cruzi were determined using orthogonal-field-alternation gel electrophoresis. At least 15 different chromosome-sized DNA molecules, ranging in size from less than 200 kilobase pairs to greater than 2000 kilobase pairs, were resolved for each of the isolates examined. Many of the bands were present in different relative intensities suggesting that the number of individual chromosomes per organism may be considerably higher. Significant inter- and intra-strain differences in molecular karyotype and in the chromosomal locations of the genes for the spliced leader, tubulins, 5S ribosomal RNA and a heat shock protein were found. These marked chromosomal differences among
T. cruzi strains and clones may be related to the high degree of phenotypic heterogeneity previously found in this parasite.
Details
- Title: Subtitle
- Trypanosoma cruzi exhibits inter- and intra-strain heterogeneity in molecular karyotype and chromosomal gene location
- Creators
- David M Engman - University of IowaLekkala V Reddy - University of IowaJohn E Donelson - University of IowaLouis V Kirchhoff - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Molecular and biochemical parasitology, Vol.22(2), pp.115-123
- Publisher
- Elsevier B.V
- DOI
- 10.1016/0166-6851(87)90041-7
- PMID
- 3553931
- ISSN
- 0166-6851
- eISSN
- 1872-9428
- Language
- English
- Date published
- 1987
- Academic Unit
- Psychiatry; Infectious Diseases; Epidemiology; Biochemistry and Molecular Biology; Internal Medicine
- Record Identifier
- 9984280869902771
Metrics
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