Journal article
Tumor Hypoxia and CD8 + T‐Cell Infiltration in Patients With Advanced Laryngeal Cancer
Otolaryngology-head and neck surgery, Vol.173(3), pp.645-650
09/2025
DOI: 10.1002/ohn.1291
PMCID: PMC12354137
PMID: 40323140
Abstract
Objective
We assessed correlations between tumor carbonic anhydrase IX (CAIX) staining, as a marker of tumor hypoxia, and CD8+ T-cell infiltration in a cohort of patients with advanced laryngeal squamous cell carcinoma undergoing a bioselection approach for definitive treatment.
Study Design
Retrospective cohort study.
Setting
Tertiary care hospital.
Methods
Patients with stage III to IV laryngeal squamous cell carcinoma treated under a bioselection paradigm were included. Immunohistochemistry for CD8+ T-cells and CAIX was performed. Nonparametric tests and Kaplan-Meier survival analyses were used to compare tumor CAIX status by clinicopathologic variables and CD8+ T-cell infiltration and to evaluate the role of CAIX and combination CAIX/tumor infiltrating lymphocytes (TIL) category on survival.
Results
Our cohort included 92 patients (n = 68 [73.9%] supraglottic). No difference in CAIX staining was seen by tumor subsite, stage, and response to induction chemotherapy (all P > .05). Thirteen (14.1%) tumors were CAIX-positive and showed significantly lower CD8+ T-cell infiltration than CAIX-negative tumors (18 [0-62] vs 32 [0-399], P = .028). Combination CAIX/TIL category was significantly associated with the likelihood of response (CAIX−/TIL[high] were less likely to respond) and in the group of responders, was predictive of a higher degree of tumor shrinkage (>80%).
Conclusion
CAIX staining correlates with reduced CD8+ T-cell infiltration in patients with advanced laryngeal squamous cell carcinoma undergoing bioselection. The combination CAIX/TIL category is associated with the likelihood and degree of response to induction. The utility of CAIX status and other combination immune and hypoxia signatures as a biomarker of induction response and survival merits prospective evaluation.
Details
- Title: Subtitle
- Tumor Hypoxia and CD8 + T‐Cell Infiltration in Patients With Advanced Laryngeal Cancer
- Creators
- Joshua D. Smith - University of Pittsburgh Medical CenterElizabeth Gensterblum-Miller - University of MichiganDavid W.A. Forner - University of MichiganPratyusha Yalamanchi - University of MichiganMarisa R. Buchakjian - University of MichiganSteven B. Chinn - University of MichiganAndrew G. Shuman - University of MichiganKeith A. Casper - University of MichiganKelly M. Malloy - University of MichiganChaz L. Stucken - University of MichiganScott A. Mclean - University of MichiganMichelle L. Mierzwa - University of MichiganJennifer Shah - University of MichiganPaul L. Swiecicki - University of MichiganFrancis P. Worden - University of MichiganMark E.P. Prince - University of MichiganMatthew E. Spector - University of Pittsburgh Medical CenterJ. Chad Brenner - University of MichiganMolly E. Heft Neal - University of Michigan
- Resource Type
- Journal article
- Publication Details
- Otolaryngology-head and neck surgery, Vol.173(3), pp.645-650
- DOI
- 10.1002/ohn.1291
- PMID
- 40323140
- PMCID
- PMC12354137
- NLM abbreviation
- Otolaryngol Head Neck Surg
- ISSN
- 0194-5998
- eISSN
- 1097-6817
- Publisher
- Wiley
- Language
- English
- Electronic publication date
- 05/05/2025
- Date published
- 09/2025
- Academic Unit
- Otolaryngology
- Record Identifier
- 9984821343002771
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