Tumor Necrosis Factor Receptor 1 Expression Is Upregulated in Dendritic Cells in Patients with Chronic HCV Who Respond to Therapy

Raul Cubillas; Katherine Kintner; Frances Phillips; Nitin J Karandikar; Dwain L Thiele; Geri R Brown

doi:10.1155/2010/429243

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Tumor Necrosis Factor Receptor 1 Expression Is Upregulated in Dendritic Cells in Patients with Chronic HCV Who Respond to Therapy

Journal article

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Peer reviewed

Tumor Necrosis Factor Receptor 1 Expression Is Upregulated in Dendritic Cells in Patients with Chronic HCV Who Respond to Therapy

Raul Cubillas, Katherine Kintner, Frances Phillips, Nitin J Karandikar, Dwain L Thiele and Geri R Brown

Hepatitis research and treatment, Vol.2010, pp.429243-10

2010

DOI: 10.1155/2010/429243

PMCID: PMC2989713

PMID: 21152182

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Abstract

The present studies assessed the level of tumor necrosis factor receptor (TNFR) expression in peripheral blood mononuclear cells (PBMCs) subsets from patients with chronic HCV undergoing interferon α /ribavirin-based therapy (Ifn/R). Methods . TNFR family member mRNA expression was determined using quantitative real-time PCR assays (RTPCRs) in PBMC from 39 HCV+ patients and 21 control HCV− patients. Further subset analysis of HCV + patients (untreated (U), sustained virological responders (SVR), and nonresponders (NR)/relapsers (Rel)) PBMC was performed via staining with anti-CD123, anti-CD33, anti-TNFR1 or via RTPCR for TNFR1 mRNA. Results . A similar level of TNFR1 mRNA in PBMC from untreated HCV+ genotype 1 patients and controls was noted. TNFR1 and TNFR2 mRNA levels in PBMC from HCV+ patients with SVR were statistically different than levels in HCV(−) patients. A significant difference was noted between the peak values of TNFR1 of the CD123+ PBMC isolated from SVR and the NR/Rel. Conclusion . Upregulation of TNFR1 expression, occurring in a specific subset of CD123+ dendritic cells, appeared in HCV+ patients with SVR.

Clinical Study

Details

Title: Subtitle: Tumor Necrosis Factor Receptor 1 Expression Is Upregulated in Dendritic Cells in Patients with Chronic HCV Who Respond to Therapy
Creators: Raul Cubillas - Division of Digestive and Liver Diseases, Department of Internal Medicine, Southwestern Medical Center at Dallas, University of Texas at Dallas, Dallas, TX 75390-9151, USA
Katherine Kintner - Division of Digestive and Liver Diseases, Department of Internal Medicine, Southwestern Medical Center at Dallas, University of Texas at Dallas, Dallas, TX 75390-9151, USA
Frances Phillips - Dallas Veterans Affairs Medical Center, 4500 S. Lancaster Road, Dallas, Texas 75216, USA
Nitin J Karandikar - Division of Digestive and Liver Diseases, Department of Internal Medicine, Southwestern Medical Center at Dallas, University of Texas at Dallas, Dallas, TX 75390-9151, USA
Dwain L Thiele - Division of Digestive and Liver Diseases, Department of Internal Medicine, Southwestern Medical Center at Dallas, University of Texas at Dallas, Dallas, TX 75390-9151, USA
Geri R Brown - Division of Digestive and Liver Diseases, Department of Internal Medicine, Southwestern Medical Center at Dallas, University of Texas at Dallas, Dallas, TX 75390-9151, USA
Resource Type: Journal article
Publication Details: Hepatitis research and treatment, Vol.2010, pp.429243-10
DOI: 10.1155/2010/429243
PMID: 21152182
PMCID: PMC2989713
NLM abbreviation: Hepat Res Treat
ISSN: 2090-1364
eISSN: 2090-1372
Publisher: Hindawi Publishing Corporation
Language: English
Date published: 2010
Academic Unit: Pathology
Record Identifier: 9984047795002771

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