Tumor necrosis factor alpha activates human immunodeficiency virus type 1 through induction of nuclear factor binding to the NF-kappa B sites in the long terminal repeat

Elia J Duh; Wendy J Maury; Thomas M Folks; Anthony S Fauci; Arnold B Rabson

doi:10.1073/pnas.86.15.5974

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Tumor necrosis factor alpha activates human immunodeficiency virus type 1 through induction of nuclear factor binding to the NF-kappa B sites in the long terminal repeat

Journal article

Open access

Peer reviewed

Tumor necrosis factor alpha activates human immunodeficiency virus type 1 through induction of nuclear factor binding to the NF-kappa B sites in the long terminal repeat

Elia J Duh, Wendy J Maury, Thomas M Folks, Anthony S Fauci and Arnold B Rabson

Proceedings of the National Academy of Sciences - PNAS, Vol.86(15), pp.5974-5978

08/1989

DOI: 10.1073/pnas.86.15.5974

PMCID: PMC297754

PMID: 2762307

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Abstract

Expression of human immunodeficiency virus type 1 (HIV-1) can be activated in a chronically infected T-cell line (ACH2 cells) by a cytokine, human tumor necrosis factor alpha (TNF-alpha). TNF-alpha treatment of ACH2 cells resulted in an increase in steady-state levels of HIV RNA and HIV transcription. Gel mobility shift assays demonstrated that the transcriptional activation of the HIV long terminal repeat (LTR) by TNF-alpha was associated with the induction of a nuclear factor(s) binding to the NF-kappa B sites in the LTR. Deletion of the NF-kappa B sites from the LTR eliminated activation by TNF-alpha in T cells transfected with plasmids in which the HIV LTR directed the expression of the bacterial chloramphenicol acetyltransferase gene. Thus, TNF-alpha appears to activate HIV RNA and virus production by ACH2 cells through the induction of transcription-activating factors that bind to the NF-kappa B sequences in the HIV LTR.

Transfection

Cell Line

HIV-1 - drug effects

Humans

Regulatory Sequences, Nucleic Acid

DNA, Viral - metabolism

Molecular Sequence Data

Nuclear Proteins - metabolism

DNA-Binding Proteins - genetics

HIV-1 - genetics

DNA-Binding Proteins - metabolism

Tumor Necrosis Factor-alpha - pharmacology

Genes, Viral - drug effects

HIV-1 - growth & development

Base Sequence

Protein Binding

Repetitive Sequences, Nucleic Acid

DNA, Viral - genetics

Virus Activation

Details

Title: Subtitle: Tumor necrosis factor alpha activates human immunodeficiency virus type 1 through induction of nuclear factor binding to the NF-kappa B sites in the long terminal repeat
Creators: Elia J Duh - Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, Bethesda, MD 20892
Wendy J Maury
Thomas M Folks
Anthony S Fauci
Arnold B Rabson
Resource Type: Journal article
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.86(15), pp.5974-5978
DOI: 10.1073/pnas.86.15.5974
PMID: 2762307
PMCID: PMC297754
NLM abbreviation: Proc Natl Acad Sci U S A
ISSN: 0027-8424
eISSN: 1091-6490
Publisher: National Academy of Sciences
Language: English
Date published: 08/1989
Academic Unit: Microbiology and Immunology
Record Identifier: 9984083227002771

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