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Twenty Years of Calcium Imaging: Cell Physiology to Dye For
Journal article   Open access

Twenty Years of Calcium Imaging: Cell Physiology to Dye For

Harm Knot, Ismail Laher, Eric Sobie, Silvia Guatimosim, Leticia Gomez-Viquez, Hali Hartmann, Long-Sheng Song, W Lederer, Wolfgang Graier, Roland Malli, …
Molecular interventions, Vol.5(2), pp.112-127
04/01/2005
DOI: 10.1124/mi.5.2.8
PMCID: PMC4861218
PMID: 15821159
url
https://www.ncbi.nlm.nih.gov/pmc/articles/4861218View
Open Access

Abstract

The use of fluorescent dyes over the past two decades has led to a revolution in our understanding of calcium signaling. Given the ubiquitous role of Ca super(2+) in signal transduction at the most fundamental levels of molecular, cellular, and organismal biology, it has been challenging to understand how the specificity and versatility of Ca super(2+) signaling is accomplished. In excitable cells, the coordination of changing Ca super(2+) concentrations at global (cellular) and well-defined subcellular spaces through the course of membrane depolarization can now be conceptualized in the context of disease processes such as cardiac arrhythmogenesis. The spatial and temporal dimensions of Ca super(2+) signaling are similarly important in non-excitable cells, such as endothelial and epithelial cells, to regulate multiple signaling pathways that participate in organ homeostasis as well as cellular organization and essential secretory processes.

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