Journal article
Twitch-related and rhythmic activation of the developing cerebellar cortex
Journal of neurophysiology, Vol.114(3), pp.1746-1756
09/2015
DOI: 10.1152/jn.00284.2015
PMCID: PMC4571769
PMID: 26156383
Abstract
The cerebellum is a critical sensorimotor structure that exhibits protracted postnatal development in mammals. Many aspects of cerebellar circuit development are activity dependent, but little is known about the nature and sources of the activity. Based on previous findings in 6-day-old rats, we proposed that myoclonic twitches, the spontaneous movements that occur exclusively during active sleep (AS), provide generalized as well as topographically precise activity to the developing cerebellum. Taking advantage of known stages of cerebellar cortical development, we examined the relationship between Purkinje cell activity (including complex and simple spikes), nuchal and hindlimb EMG activity, and behavioral state in unanesthetized 4-, 8-, and 12-day-old rats. AS-dependent increases in complex and simple spike activity peaked at 8 days of age, with 60% of units exhibiting significantly more activity during AS than wakefulness. Also, at all three ages, approximately one-third of complex and simple spikes significantly increased their activity within 100 ms of twitches in one of the two muscles from which we recorded. Finally, we observed rhythmicity of complex and simple spikes that was especially prominent at 8 days of age and was greatly diminished by 12 days of age, likely due to developmental changes in climbing fiber and mossy fiber innervation patterns. All together, these results indicate that the neurophysiological activity of the developing cerebellum can be used to make inferences about changes in its microcircuitry. They also support the hypothesis that sleep-related twitches are a prominent source of discrete climbing and mossy fiber activity that could contribute to the activity-dependent development of this critical sensorimotor structure.
Details
- Title: Subtitle
- Twitch-related and rhythmic activation of the developing cerebellar cortex
- Creators
- Greta Sokoloff - Department of Psychological and Brain Sciences, University of Iowa, Iowa City, Iowa; DeLTA Center, University of Iowa, Iowa City, Iowa; greta-sokoloff@uiowa.eduAlan M Plumeau - Interdisciplinary Program in Neuroscience, University of Iowa, Iowa City, Iowa; andDidhiti Mukherjee - Department of Psychological and Brain Sciences, University of Iowa, Iowa City, Iowa; DeLTA Center, University of Iowa, Iowa City, IowaMark S Blumberg - Department of Psychological and Brain Sciences, University of Iowa, Iowa City, Iowa; DeLTA Center, University of Iowa, Iowa City, Iowa; Department of Biology, University of Iowa, Iowa City, Iowa
- Resource Type
- Journal article
- Publication Details
- Journal of neurophysiology, Vol.114(3), pp.1746-1756
- Publisher
- United States
- DOI
- 10.1152/jn.00284.2015
- PMID
- 26156383
- PMCID
- PMC4571769
- ISSN
- 0022-3077
- eISSN
- 1522-1598
- Grant note
- R01 HD063071 / NICHD NIH HHS\nR37-HD-81168 / NICHD NIH HHS\nR37 HD081168 / NICHD NIH HHS
- Language
- English
- Date published
- 09/2015
- Academic Unit
- Psychological and Brain Sciences; Iowa Neuroscience Institute; Biology
- Record Identifier
- 9984070739402771
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