Journal article
Two-Dimensional Fluorescence Correlation in Capillary Electrophoresis for Peak Resolution and Species Identification
Analytical chemistry (Washington), Vol.72(19), pp.4531-4542
10/01/2000
DOI: 10.1021/ac000534i
PMID: 11028607
Abstract
A new spectroscopic dimension - fluorescence intensity correlation - is introduced to enhance peak resolution and species identification in capillary electrophoresis. In two-dimensional correlation CE, a conventional electropherogram is spread into two dimensions through cross-correlation analysis of fluorescence time response. A laser that is sinusoidally modulated in intensity is used as the excitation source. Three channels of information are collected during a CE run: the steady-state intensity, the ac amplitude, and the phase-resolved fluorescence intensity. The correlation between two chosen channels is then evaluated. A two-dimensional correlation electropherogram consists of a plot of the correlation intensity versus two axes of migration time. Through correlation analysis, species discrimination and peak resolution are significantly enhanced without having to physically separate the solutes. Two-dimensional correlation CE showed complete resolution between two overlapping sample peaks with a resolution of 0.28 in the conventional one-dimensional electropherogram. In separations of polycyclic aromatic hydrocarbons by micellar electrokinetic chromatography (MEKC), two-dimensional correlation analysis resolved all overlapping elution peaks unseparable by one-dimensional MEKC, demonstrating the utility of 2D correlation in separation method development. The capability of 2D correlation CE in species identification is demonstrated with a sequence of 39 consecutively injected peaks containing four fluorescent dyes. Species identification in sequencing is achieved without complex data treatment in two-dimensional correlation CE.
Details
- Title: Subtitle
- Two-Dimensional Fluorescence Correlation in Capillary Electrophoresis for Peak Resolution and Species Identification
- Creators
- Gufeng Wang - Department of Chemistry, University of Iowa, Iowa City, Iowa 52242Lei Geng - Department of Chemistry, University of Iowa, Iowa City, Iowa 52242
- Resource Type
- Journal article
- Publication Details
- Analytical chemistry (Washington), Vol.72(19), pp.4531-4542
- DOI
- 10.1021/ac000534i
- PMID
- 11028607
- ISSN
- 0003-2700
- eISSN
- 1520-6882
- Language
- English
- Date published
- 10/01/2000
- Academic Unit
- Chemistry
- Record Identifier
- 9984216693202771
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