Two NFAT Transcription Factor Binding Sites Participate in the Regulation of CD95 (Fas) Ligand Expression in Activated Human T Cells

Kevin M. Latinis; Lyse A. Norian; Steve L. Eliason; Gary A. Koretzky

doi:10.1074/jbc.272.50.31427

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Two NFAT Transcription Factor Binding Sites Participate in the Regulation of CD95 (Fas) Ligand Expression in Activated Human T Cells

Journal article

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Two NFAT Transcription Factor Binding Sites Participate in the Regulation of CD95 (Fas) Ligand Expression in Activated Human T Cells

Kevin M. Latinis, Lyse A. Norian, Steve L. Eliason and Gary A. Koretzky

The Journal of biological chemistry, Vol.272(50), pp.31427-31434

12/12/1997

DOI: 10.1074/jbc.272.50.31427

PMID: 9395475

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Abstract

Antigen receptor engagement on T lymphocytes activates transcription factors important for stimulating cytokine gene expression. This is critical for clonal expansion of antigen-specific T cells and propagation of immune responses. Additionally, under some conditions antigen receptor stimulation initiates apoptosis of T lymphocytes through the induced expression of CD95 ligand and its receptor. Here we demonstrate that the transcription factor, NFAT, which is critical for the inducible expression of many cytokine genes, also plays a critical role in the regulation of T cell receptor-mediated CD95 ligand expression. Two sites within the CD95 ligand promoter, identified through DNase I footprinting, bind NFAT proteins from nuclear extracts of activated T cells. Although both sites appear important for optimal expression of CD95 ligand in activated T cells, mutational analysis suggests that the distal NFAT site plays a more significant role. Furthermore, these sites do not appear to be required for constitutive CD95 ligand expression in Sertoli cells.

Details

Title: Subtitle: Two NFAT Transcription Factor Binding Sites Participate in the Regulation of CD95 (Fas) Ligand Expression in Activated Human T Cells
Creators: Kevin M. Latinis - University of Iowa
Lyse A. Norian - University of Iowa
Steve L. Eliason - University of Iowa
Gary A. Koretzky - University of Iowa
Resource Type: Journal article
Publication Details: The Journal of biological chemistry, Vol.272(50), pp.31427-31434
Publisher: Elsevier Inc
DOI: 10.1074/jbc.272.50.31427
PMID: 9395475
ISSN: 0021-9258
eISSN: 1083-351X
Language: English
Date published: 12/12/1997
Academic Unit: Anatomy and Cell Biology
Record Identifier: 9984622757202771

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