Journal article
Two-Photon Polymerization as a Tool for Studying 3D Printed Topography-Induced Stem Cell Fate
Macromolecular bioscience, Vol.19(2), pp.e1800370-n/a
02/2019
DOI: 10.1002/mabi.201800370
PMCID: PMC6365162
PMID: 30430755
Abstract
Geometric topographies are known to influence cellular differentiation toward specific phenotypes, but to date the range of features and type of substrates that can be easily fabricated to study these interactions is somewhat limited. In this study, an emerging technology, two-photon polymerization, is used to print topological patterns with varying feature-size and thereby study their effect on cellular differentiation. This technique offers rapid manufacturing of topographical surfaces with good feature resolution for shapes smaller than 3 µm. Human-induced pluripotent stem cells, when attached to these substrates or a non-patterned control for 1 week, express an array of genetic markers that suggest their differentiation toward a heterogeneous population of multipotent progenitors from all three germ layers. Compared to the topographically smooth control, small features (1.6 µm) encourage differentiation toward ectoderm while large features (8 µm) inhibit self-renewal. This study demonstrates the potential of using two-photon polymerization to study and control stem cell fate as a function of substrate interactions. The ability to tailor and strategically design biomaterials in this way can enable more precise and efficient generation or maintenance of desired phenotypes in vitro and in vivo.
Details
- Title: Subtitle
- Two-Photon Polymerization as a Tool for Studying 3D Printed Topography-Induced Stem Cell Fate
- Creators
- Kristan S Worthington - Department of Biomedical Engineering, College of Engineering, The University of Iowa, Iowa City, IA, 52242, USAAnh-Vu Do - Department of Pharmaceutics and Translational Therapeutics, College of Pharmacy, The University of Iowa, Iowa City, IA, 52242, USARasheid Smith - Department of Pharmaceutics and Translational Therapeutics, College of Pharmacy, The University of Iowa, Iowa City, IA, 52242, USABudd A Tucker - Institute for Vision Research, Department of Ophthalmology and Visual Science, Carver College of Medicine, The University of Iowa, Iowa City, IA, 52242, USAAliasger K Salem - Department of Pharmaceutics and Translational Therapeutics, College of Pharmacy, The University of Iowa, Iowa City, IA, 52242, USA
- Resource Type
- Journal article
- Publication Details
- Macromolecular bioscience, Vol.19(2), pp.e1800370-n/a
- DOI
- 10.1002/mabi.201800370
- PMID
- 30430755
- PMCID
- PMC6365162
- NLM abbreviation
- Macromol Biosci
- ISSN
- 1616-5187
- eISSN
- 1616-5195
- Publisher
- Germany
- Grant note
- P30 ES005605 / NIEHS NIH HHS R01 EY024605 / NEI NIH HHS 18-5045 / Roy J. Carver Charitable Trust 5P30CA086862 / NIH HHS 1 R01 024605-01 / NIH HHS P30 CA086862 / NCI NIH HHS Holden Comprehensive Cancer Center Lyle and Sharon Bighley Chair of Pharmaceutical Sciences 1 S10 RR022498-01 / Roy J. Carver Charitable Trust Roy J. and Lucille A. Carver College of Medicine at the University of Iowa S10 RR022498 / NCRR NIH HHS Office of the Vice President for Research and Economic Development DP2 OD007483 / NIH HHS 1 DP2 OD007483-01 / NIH HHS
- Language
- English
- Date published
- 02/2019
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Iowa Neuroscience Institute; Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Dental Research; Chemical and Biochemical Engineering; Ophthalmology and Visual Sciences
- Record Identifier
- 9983985918802771
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