Journal article
Two distinct functions of Lim1 in the Drosophila antenna
microPublication biology, Vol.2024(6)
06/17/2024
DOI: 10.17912/micropub.biology.001229
PMCID: PMC11217802
PMID: 38957438
Abstract
The Lim1 transcription factor is required in Drosophila for patterning the eye-antennal disk. At the adult stage, Lim1 is strongly expressed in Johnston's Organ (JO) neurons, the antennal auditory organ. Using RNAi-mediated knockdown of Lim1 using a strong neuronal driver, we find a significant reduction in electrophysiological responses to auditory stimuli, recorded from the antennal nerve. This reduction can be accounted for by Lim1 knockdown in the auditory subset of JO neurons, with no effect of knockdown in JO neuron subsets associated with wind or gravity detection. Conversely, Lim1 knockdown in JO sense organ precursors had no effect on hearing. Mosaic animals with antennal clones of the Lim1 E9 null mutation showed morphological defects in the antenna, and significant auditory electrophysiological defects. Our results are consistent with two distinct functions for Lim1 in the antenna, including an early patterning function in the eye-antennal disk, and a later neural differentiation function in the JO neurons.
Details
- Title: Subtitle
- Two distinct functions of Lim1 in the Drosophila antenna
- Creators
- Dylan M Dolezal - University of IowaMei-ling A Joiner - University of IowaDaniel F Eberl - Biology, University of Iowa, Iowa City, Iowa, United States
- Resource Type
- Journal article
- Publication Details
- microPublication biology, Vol.2024(6)
- DOI
- 10.17912/micropub.biology.001229
- PMID
- 38957438
- PMCID
- PMC11217802
- NLM abbreviation
- MicroPubl Biol
- ISSN
- 2578-9430
- eISSN
- 2578-9430
- Publisher
- Caltech Library
- Language
- English
- Date published
- 06/17/2024
- Academic Unit
- Iowa Neuroscience Institute; Biology
- Record Identifier
- 9984651259802771
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