Journal article
Two rigor states in skinned crayfish single muscle fibers
The Journal of general physiology, Vol.68(3), pp.267-280
09/1976
DOI: 10.1085/jgp.68.3.267
PMCID: PMC2228434
PMID: 821913
Abstract
We studied the tension and stiffness of crayfish skinned single muscle fibers during and after the induction of rigor by removal of MgATP (substrate). We found that the rigor state is not unique but depends on the condition of the muscle before rigor. Fibers induced into rigor with a minimum of activation (low rigor) develop a small tension and moderate stiffness, while those entering rigor during maximum activation (high rigor) maintain near peak tension (80%) and develop a high stiffness. These rigor states are insensitive to Ca addition or deletion but they are partially interconvertible by length change. Stiffness changes when the rigor muscle length is varied, a condition in which the number of attached cross-rigor muscle length is varied, a condition in which the number of attached cross-bridges cannot change, and high-rigor muscle becomes less stiff than low-rigor muscle when the former is brought to the same tension by length release. The sensitivity of low, high, or length-released high-rigor muscles to trace substrate concentration (less than muM) differs, and rigor at lower strain is more suscepitible to substrate.
Details
- Title: Subtitle
- Two rigor states in skinned crayfish single muscle fibers
- Creators
- Masataka KawaiPhilip W Brandt
- Resource Type
- Journal article
- Publication Details
- The Journal of general physiology, Vol.68(3), pp.267-280
- DOI
- 10.1085/jgp.68.3.267
- PMID
- 821913
- PMCID
- PMC2228434
- NLM abbreviation
- J Gen Physiol
- ISSN
- 0022-1295
- eISSN
- 1540-7748
- Publisher
- United States
- Language
- English
- Date published
- 09/1976
- Academic Unit
- Anatomy and Cell Biology; Internal Medicine
- Record Identifier
- 9984025589202771
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