Two types of K(+) channel subunit, Erg1 and KCNQ2/3, contribute to the M-like current in a mammalian neuronal cell

A A Selyanko; J K Hadley; I C Wood; F C Abogadie; P Delmas; N J Buckley; B London; D A Brown

doi:10.1523/JNEUROSCI.19-18-07742.1999

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Two types of K(+) channel subunit, Erg1 and KCNQ2/3, contribute to the M-like current in a mammalian neuronal cell

Journal article

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Peer reviewed

Two types of K(+) channel subunit, Erg1 and KCNQ2/3, contribute to the M-like current in a mammalian neuronal cell

A A Selyanko, J K Hadley, I C Wood, F C Abogadie, P Delmas, N J Buckley, B London and D A Brown

The Journal of neuroscience, Vol.19(18), pp.7742-7756

09/15/1999

DOI: 10.1523/JNEUROSCI.19-18-07742.1999

PMCID: PMC6782456

PMID: 10479678

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Abstract

The potassium M current was originally identified in sympathetic ganglion cells, and analogous currents have been reported in some central neurons and also in some neural cell lines. It has recently been suggested that the M channel in sympathetic neurons comprises a heteromultimer of KCNQ2 and KCNQ3 (Wang et al., 1998) but it is unclear whether all other M-like currents are generated by these channels. Here we report that the M-like current previously described in NG108-15 mouse neuroblastoma x rat glioma cells has two components, "fast" and "slow", that may be differentiated kinetically and pharmacologically. We provide evidence from PCR analysis and expression studies to indicate that these two components are mediated by two distinct molecular species of K(+) channel: the fast component resembles that in sympathetic ganglia and is probably carried by KCNQ2/3 channels, whereas the slow component appears to be carried by merg1a channels. Thus, the channels generating M-like currents in different cells may be heterogeneous in molecular composition.

Cell Differentiation

Transfection

Potassium Channels, Voltage-Gated

Humans

Hybrid Cells

Potassium Channels - physiology

Neurons - cytology

Neuroblastoma

Superior Cervical Ganglion - physiology

KCNQ3 Potassium Channel

Receptors, Muscarinic - drug effects

KCNQ2 Potassium Channel

Neurons - physiology

Indoles - pharmacology

CHO Cells

Membrane Potentials - drug effects

Cricetinae

Mice, Inbred C57BL

Rats

Membrane Potentials - physiology

Receptor, Muscarinic M1

Animals

Glioma

Benzimidazoles - pharmacology

Mice

Pyridines - pharmacology

Kinetics

Receptors, Muscarinic - physiology

Sulfanilamides - pharmacology

Details

Title: Subtitle: Two types of K(+) channel subunit, Erg1 and KCNQ2/3, contribute to the M-like current in a mammalian neuronal cell
Creators: A A Selyanko - Department of Pharmacology, University College London, London, WC1E 6BT, United Kingdom
J K Hadley
I C Wood
F C Abogadie
P Delmas
N J Buckley
B London
D A Brown
Resource Type: Journal article
Publication Details: The Journal of neuroscience, Vol.19(18), pp.7742-7756
Publisher: United States
DOI: 10.1523/JNEUROSCI.19-18-07742.1999
PMID: 10479678
PMCID: PMC6782456
ISSN: 0270-6474
eISSN: 1529-2401
Grant note: Wellcome Trust
Language: English
Date published: 09/15/1999
Academic Unit: Molecular Physiology and Biophysics; Cardiovascular Medicine; Internal Medicine
Record Identifier: 9984025435202771

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