Type 1 diabetes alters ischemia-induced gene expression

Rahul Peravali; Lucas Gunnels; Satyanarayana Alleboina; Ivan C. Gerling; Ayotunde O. Dokun

doi:10.1016/j.jcte.2018.11.003

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Type 1 diabetes alters ischemia-induced gene expression

Journal article

Open access

Peer reviewed

Type 1 diabetes alters ischemia-induced gene expression

Rahul Peravali, Lucas Gunnels, Satyanarayana Alleboina, Ivan C. Gerling and Ayotunde O. Dokun

Journal of clinical & translational endocrinology, Vol.15, pp.19-24

11/22/2018

DOI: 10.1016/j.jcte.2018.11.003

PMCID: PMC6279996

PMID: 30555789

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Abstract

Peripheral Artery Disease (PAD) is a chronic, activity-limiting disease that is caused by atherosclerotic occlusion of blood vessels outside the heart. Type 1 Diabetes (T1D) not only increases an individual’s likelihood of developing PAD, but also contributes to poor clinical outcomes after PAD manifestation. Although there is some evidence suggesting that hyperglycemia might alter expression of genes involved in regulating PAD severity or outcomes, our knowledge about the specific genes and pathways involved remains incomplete. We induced experimental PAD or hind limb ischemia in T1D and non-diabetic mice and subjected the ischemic gastrocnemius muscle tissues to genome-wide mRNA transcriptome and pathway analysis. We identified 513 probe sets that represented 443 different genes with highly significant expression differences (p < 0.005) between the ischemic diabetic and ischemic non-diabetic muscle tissues. Moreover, pathway analysis of the differentially expressed genes identified pathways involved in essential biological processes such as “cell cycle,” “DNA replication,” “metabolic pathways,” “focal adhesion,” “regulation of actin cytoskeleton,” and “nucleotide excision repair”. Taken together, our data offer the opportunity to test hypotheses on the roles played by the altered genes/molecular pathways in poor PAD outcomes in diabetes. Such studies may lead to the development of specific therapies to improve PAD outcomes in patients with comorbid diabetes.

Genome-wide gene expression profiling

Molecular pathway analysis

Peripheral artery disease

Research Paper

Type 1 diabetes mellitus

Details

Title: Subtitle: Type 1 diabetes alters ischemia-induced gene expression
Creators: Rahul Peravali - University of Tennessee Health Science Center
Lucas Gunnels - University of Tennessee Health Science Center
Satyanarayana Alleboina - University of Tennessee Health Science Center
Ivan C. Gerling - University of Tennessee Health Science Center
Ayotunde O. Dokun - University of Tennessee Health Science Center
Resource Type: Journal article
Publication Details: Journal of clinical & translational endocrinology, Vol.15, pp.19-24
DOI: 10.1016/j.jcte.2018.11.003
PMID: 30555789
PMCID: PMC6279996
NLM abbreviation: J Clin Transl Endocrinol
ISSN: 2214-6237
eISSN: 2214-6237
Publisher: Elsevier
Grant note: DOI: 10.13039/100000050, name: National Heart, Lung, and Blood Institute, award: R01 HL130399
Language: English
Date published: 11/22/2018
Academic Unit: Molecular Physiology and Biophysics; Fraternal Order of Eagles Diabetes Research Center; Endocrinology and Metabolism; Internal Medicine
Record Identifier: 9984297498502771

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