Journal article
Type 3 Fimbriae and Biofilm Formation Are Regulated by the Transcriptional Regulators MrkHI in Klebsiella pneumoniae
Journal of bacteriology, Vol.193(14), pp.3453-3460
07/01/2011
DOI: 10.1128/JB.00286-11
PMCID: PMC3133326
PMID: 21571997
Abstract
Klebsiella pneumoniae is an opportunistic pathogen which frequently causes hospital-acquired urinary and respiratory tract infections. K. pneumoniae may establish these infections in vivo following adherence, using the type 3 fimbriae, to indwelling devices coated with extracellular matrix components. Using a colony immunoblot screen, we identified transposon insertion mutants which were deficient for type 3 fimbrial surface production. One of these mutants possessed a transposon insertion within a gene, designated mrkI, encoding a putative transcriptional regulator. A site-directed mutant of this gene was constructed and shown to be deficient for fimbrial surface expression under aerobic conditions. MrkI mutants have a significantly decreased ability to form biofilms on both abiotic and extracellular matrix-coated surfaces. This gene was found to be cotranscribed with a gene predicted to encode a PilZ domain-containing protein, designated MrkH. This protein was found to bind cyclic-di-GMP (c-di-GMP) and regulate type 3 fimbrial expression.
Details
- Title: Subtitle
- Type 3 Fimbriae and Biofilm Formation Are Regulated by the Transcriptional Regulators MrkHI in Klebsiella pneumoniae
- Creators
- Jeremiah G. Johnson - University of IowaCaitlin N. Murphy - University of IowaJean Sippy - University of IowaTylor J. Johnson - Univ Iowa, Dept Microbiol, Roy J & Lucille A Carver Coll Med, Iowa City, IA 52242 USASteven Clegg - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Journal of bacteriology, Vol.193(14), pp.3453-3460
- Publisher
- Amer Soc Microbiology
- DOI
- 10.1128/JB.00286-11
- PMID
- 21571997
- PMCID
- PMC3133326
- ISSN
- 0021-9193
- eISSN
- 1098-5530
- Number of pages
- 8
- Grant note
- R01AI050011 / NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Institute of Allergy & Infectious Diseases (NIAID) R01 AI050011; T32 AI007511 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA
- Language
- English
- Date published
- 07/01/2011
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984696726402771
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