Journal article
Type I Interferon Is the Primary Regulator of Inducible Ly-6C Expression on T Cells
Journal of interferon & cytokine research, Vol.21(8), pp.621-629
08/2001
DOI: 10.1089/10799900152547885
PMID: 11559440
Abstract
Ly-6C has been proposed as a marker of memory CD8+ T cells. Reports have indicated that Ly-6C is upregulated after T cell receptor (TCR) stimulation or exposure to proinflammatory cytokines. This study examined the relative roles of proinflammatory cytokines and TCR engagement in Ly-6C induction. In vitro experiments tested the effects of cytokines on Ly-6C expression and confirmed interferon-alpha (IFN-alpha) as a primary cytokine that induces Ly-6C expression on CD4+ and CD8+ T cells. The amount and duration of Ly-6C expression were examined on T cells after in vivo induction of proinflammatory cytokines (CpG oligodeoxynucleotides [ODN]) or TCR activation (staphylococcal enterotoxin B [SEB]). In vivo, proinflammatory cytokines transiently upregulated Ly-6C on T cells in the absence of TCR stimulation. TCR stimulation by SEB transiently upregulated Ly-6C expression on antigen-specific and antigen-nonspecific T cells but did not cause long-term upregulation of Ly-6C expression in either population. IFN-alpha was confirmed as a primary inducer of Ly-6C in vivo, as CpG ODN were unable to induce Ly-6C expression in IFN-alphaRI(-/-) mice. Thus, inducible Ly-6C expression on CD4+ and CD8+ T cells is largely due to IFN-alpha in the environment and appears not to be directly correlated with the development of T cell memory.
Details
- Title: Subtitle
- Type I Interferon Is the Primary Regulator of Inducible Ly-6C Expression on T Cells
- Creators
- Annette J Schlueter - Department of Pathology, University of Iowa College of Medicine, Iowa City, IA 52242-1181Arthur M Krieg - Coley Pharmaceutical Group, Wellesley, MA 02481Peter de Vries - Cell Therapeutics, Inc., Seattle, WA 98119Xiang Li - Department of Pathology, University of Iowa College of Medicine, Iowa City, IA 52242-1181
- Resource Type
- Journal article
- Publication Details
- Journal of interferon & cytokine research, Vol.21(8), pp.621-629
- DOI
- 10.1089/10799900152547885
- PMID
- 11559440
- ISSN
- 1079-9907
- eISSN
- 1557-7465
- Language
- English
- Date published
- 08/2001
- Academic Unit
- Pathology
- Record Identifier
- 9984047752002771
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