Journal article
Tyrosinase transfection produces melanin synthesis and growth retardation in glioma cells
Melanoma research, Vol.8(6), pp.493-498
12/01/1998
DOI: 10.1097/00008390-199812000-00003
PMID: 9918410
Abstract
Tyrosinase is the key enzyme in melanin biosynthesis in pigmented cells. We transfected 9L rat glioma cells with human tyrosinase cDNA that had been cloned in a high expression vector. Stable transfectants were selected by their resistance to the antibiotic G418, More than a dozen G418-resistant clones were isolated and were screened for tyrosinase expression using dopa-oxidase activity. The clone with the highest tyrosinase activity was selected and expanded for further studies. Northern blot analyses of total RNA from cells showed that the transfected cells had relatively more tyrosinase transcript than SK-MEL-23 human melanotic melanoma cells. The melanin content of the transfected cells was dependent on the concentration of L-tyrosine in the culture medium. In addition, the growth of transfected cells was inhibited when grown in a medium containing high concentrations of L-tyrosine. These results suggest that tyrosinase activity is cytotoxic in a substrate-dependent manner. This may have far reaching therapeutic use for glioma tumours. (C) 1998 Lippincott Williams & Wilkins.
Details
- Title: Subtitle
- Tyrosinase transfection produces melanin synthesis and growth retardation in glioma cells
- Creators
- M V Singh - University of AlbertaK Jimbow
- Resource Type
- Journal article
- Publication Details
- Melanoma research, Vol.8(6), pp.493-498
- Publisher
- Lippincott Williams & Wilkins
- DOI
- 10.1097/00008390-199812000-00003
- PMID
- 9918410
- ISSN
- 0960-8931
- eISSN
- 1473-5636
- Number of pages
- 6
- Language
- English
- Date published
- 12/01/1998
- Academic Unit
- Endocrinology and Metabolism; Internal Medicine
- Record Identifier
- 9984359796202771
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