Journal article
Tyrosine-ester sulfotransferase from rat liver: Bacterial expression and identification
Protein expression and purification, Vol.3(5), pp.421-426
1992
DOI: 10.1016/S1046-5928(05)80045-2
PMID: 1458056
Abstract
A nucleotide sequence that had been proposed for, but not identified as, rat liver aryl sulfotransferase (EC 2.8.2.1) was prepared in an appropriate vector and transformed into
Escherichia coli. The protein, expressed in large amounts, was not aryl sulfotransferase (EC 2.8.2.1) but rather tyrosine-ester sulfotransferase (EC 2.8.2.9), a sulfotransferase also active with phenols but having a much wider substrate range that includes hydroxylamines and esters of tyrosine. The recombinant tyrosine-ester sulfotransferase was identified by its unique substrate spectrum, by comparison with three peptides that were sequenced from homogeneous tyrosine-ester sulfotransferase isolated directly from rat liver, and by the specificity of antibody raised to the rat liver enzyme. Two isoforms were obtained, each of which was difficult to solubilize upon sonication of
E. coli. Both forms were solubilized with a solution of polyols (glycerol and sucrose) and subsequently purified to homogeneity.
Details
- Title: Subtitle
- Tyrosine-ester sulfotransferase from rat liver: Bacterial expression and identification
- Creators
- Xiang Chen - National Institute of Diabetes and Digestive and Kidney DiseasesYuh-Shyong Yang - National Institute of Diabetes and Digestive and Kidney DiseasesYuqun Zheng - University of IowaBrian M. Martin - National Institute of Mental HealthMichael W. Duffel - National Institute of Mental HealthWilliam B. Jakoby - National Institute of Diabetes and Digestive and Kidney Diseases
- Resource Type
- Journal article
- Publication Details
- Protein expression and purification, Vol.3(5), pp.421-426
- Publisher
- Elsevier Inc
- DOI
- 10.1016/S1046-5928(05)80045-2
- PMID
- 1458056
- ISSN
- 1046-5928
- eISSN
- 1096-0279
- Language
- English
- Date published
- 1992
- Academic Unit
- Pharmaceutical Sciences and Experimental Therapeutics; Medicinal and Natural Products Chemistry
- Record Identifier
- 9984303162702771
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