Journal article
UGGT1 retains proinsulin in the endoplasmic reticulum in an arginine dependent manner
Biochemical and biophysical research communications, Vol.527(3), pp.668-675
06/30/2020
DOI: 10.1016/j.bbrc.2020.04.158
PMCID: PMC7863631
PMID: 32423812
Abstract
We sought to clarify a pathway by which L- and dD-arginine simulate insulin secretion in mice and cell lines and obtained the following novel two findings. (1) Using affinity magnetic nanobeads technology, we identified that proinsulin is retained in the endoplasmic reticulum (ER) through UDP-glucose:glycoprotein glucosyltransferase 1 (UGGT1) when arginine availability is limited. (2) L- and d-arginine release proinsulin from UGGT1 through competition with proinsulin and promote exit of proinsulin from the ER to Golgi apparatus. The ability of arginine to release proinsulin from UGGT1 closely correlates with arginine-induced insulin secretion in several models of β cells indicating that UGGT1-proinsulin interaction regulates arginine-induced insulin secretion.
[Display omitted]
•UGGT1 binds to proinsulin in the absence of arginine.•Arginine competes with proinsulin and binds to UGGT1 in the ER.•Released proinsulin moves to Golgi apparatus and secretory vesicles to secrete.
Details
- Title: Subtitle
- UGGT1 retains proinsulin in the endoplasmic reticulum in an arginine dependent manner
- Creators
- Jaeyong Cho - National Center for Geriatrics and GerontologyMasaki Hiramoto - Tokyo Medical UniversityYuka Masaike - Tokyo Medical UniversitySatoshi Sakamoto - Tokyo Institute of TechnologyYoichi Imai - Tokyo University of ScienceYumi Imai - University of Iowa, Internal MedicineHiroshi Handa - Tokyo Medical UniversityTakeshi Imai - National Center for Geriatrics and Gerontology
- Resource Type
- Journal article
- Publication Details
- Biochemical and biophysical research communications, Vol.527(3), pp.668-675
- Publisher
- Elsevier Inc
- DOI
- 10.1016/j.bbrc.2020.04.158
- PMID
- 32423812
- PMCID
- PMC7863631
- ISSN
- 0006-291X
- eISSN
- 1090-2104
- Grant note
- DOI: 10.13039/501100001700, name: Ministry of Education, Culture, Sports, Science and Technology, award: 18659493, 18H02779, S1411011; DOI: 10.13039/501100002241, name: Japan Science and Technology Agency, award: FY2013–SICP; DOI: 10.13039/501100007312, name: National Center for Geriatrics and Gerontology, award: 28–25; DOI: 10.13039/100000002, name: National Institutes of Health, award: R01-DK090490; DOI: 10.13039/501100001691, name: Japan Society for the Promotion of Science, award: 17H06112; DOI: 10.13039/100000041, name: American Diabetes Association, award: 1-17-IBS-132
- Language
- English
- Date published
- 06/30/2020
- Academic Unit
- Endocrinology and Metabolism; Internal Medicine
- Record Identifier
- 9984359955502771
Metrics
15 Record Views