Journal article
Ubiquinol Supplementation of Donor Tissue Enhances Corneal Endothelial Cell Mitochondrial Respiration
Cornea, Vol.39(10), pp.1285-1290
10/2020
DOI: 10.1097/ICO.0000000000002408
PMID: 32558735
Abstract
To determine whether ubiquinol improves mitochondrial function and cell viability in human donor corneal endothelial cells during hypothermic corneal tissue storage.
Endothelial cell Descemet membrane tissues were treated with 10 μM ubiquinol, the reduced form of the antioxidant coenzyme Q10, for 5 days in Optisol-GS storage media before assaying for mitochondrial activity using extracellular flux analysis of oxygen consumption. In addition, endothelial cell Descemet membrane tissues were analyzed for cell viability using apoptosis and necrosis assays. Control tissues from mate corneas were treated with diluent only, and comparisons were analyzed for differences.
A total of 13 donor corneal tissues with a mean (SEM) preservation time of 11.8 days (0.4) were included for the analysis. Treatment with 10 μM ubiquinol increased spare respiratory capacity by 174% (P = 0.001), maximal respiration by 93% (P = 0.003), and proton leak by 80% (P = 0.047) compared with controls. Cells treated with ubiquinol had no significant change in cell necrosis or apoptosis.
Preliminary testing in donor corneal tissue at specified doses indicates that ubiquinol may be a useful biocompatible additive to hypothermic corneal storage media that increases corneal endothelial cell mitochondrial function. Additional investigations are indicated to further study and optimize the dose and formulation of ubiquinol for use in preserving donor corneal tissue function during hypothermic storage.
Details
- Title: Subtitle
- Ubiquinol Supplementation of Donor Tissue Enhances Corneal Endothelial Cell Mitochondrial Respiration
- Creators
- Jessica M Skeie - Iowa Lions Eye Bank, Coralville, IABenjamin T Aldrich - Iowa Lions Eye Bank, Coralville, IADarryl Y Nishimura - Iowa Lions Eye Bank, Coralville, IAGregory A Schmidt - Iowa Lions Eye Bank, Coralville, IAM Bridget Zimmerman - Department of Biostatistics, College of Public Health, University of Iowa, Iowa City, IA; andJennifer J Ling - Iowa Lions Eye Bank, Coralville, IAYoussef W Naguib - Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, University of Iowa, Iowa City, IAAliasger K Salem - Department of Pharmaceutical Sciences and Experimental Therapeutics, College of Pharmacy, University of Iowa, Iowa City, IAMark A Greiner - Iowa Lions Eye Bank, Coralville, IA
- Resource Type
- Journal article
- Publication Details
- Cornea, Vol.39(10), pp.1285-1290
- DOI
- 10.1097/ICO.0000000000002408
- PMID
- 32558735
- NLM abbreviation
- Cornea
- ISSN
- 0277-3740
- eISSN
- 1536-4798
- Grant note
- P30 ES005605 / NIEHS NIH HHS
- Language
- English
- Date published
- 10/2020
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Pharmaceutical Sciences and Experimental Therapeutics; Biostatistics; Craniofacial Anomalies Research Center; Dental Research; Chemical and Biochemical Engineering; Ophthalmology and Visual Sciences
- Record Identifier
- 9984111973702771
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