Journal article
Ultrarapid staining for cutaneous melanoma: study and protocol
Dermatologic surgery, Vol.31(7 Pt 1), pp.753-757
07/2005
DOI: 10.1097/00042728-200507000-00004
PMID: 16029700
Abstract
Immunohistochemistry has revolutionized the use of frozen sections for the removal of melanoma.
Two studies were performed to address the best and fastest way to stain melanocytes in frozen tissue sections.
Fifty-six standing cones were taken from sun-damaged white skin and stained with combinations of anti-S-100, anti-HMB-45, anti-Melan-A, and azure B with appropriate controls.
Anti-Melan-A was the best immunohistochemical stain. Azure B stains melanin granules metachromatically green and allows differentiation of melanocytes and melanophages. Ultrarapid staining worked as well as rapid staining.
Dako's EnVision ultrarapid staining protocol and azure B counterstain represent a dramatic improvement and should be considered the easily obtainable histologic standard when excising cutaneous melanoma.
Details
- Title: Subtitle
- Ultrarapid staining for cutaneous melanoma: study and protocol
- Creators
- Daniel A Davis - Department of Dermatology, University of Arkansas for the Medical Sciences, Little Rock, AR 52205, USA. davisdaniela@uams.eduKevin A KurtzRobert A Robinson
- Resource Type
- Journal article
- Publication Details
- Dermatologic surgery, Vol.31(7 Pt 1), pp.753-757
- Publisher
- United States
- DOI
- 10.1097/00042728-200507000-00004
- PMID
- 16029700
- ISSN
- 1076-0512
- eISSN
- 1524-4725
- Language
- English
- Date published
- 07/2005
- Academic Unit
- Oral Pathology, Radiology and Medicine; Pathology
- Record Identifier
- 9984047781302771
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