Journal article
Ultrasound Enhanced Matrix Metalloproteinase-9 Triggered Release of Contents from Echogenic Liposomes
Molecular pharmaceutics, Vol.9(9), pp.2554-2564
08/15/2012
DOI: 10.1021/mp300165s
PMCID: PMC3433587
PMID: 22849291
Abstract
The extracellular enzyme matrix metalloproteinase-9 (MMP-9) is overexpressed in atherosclerotic plaques and in metastatic cancers. The enzyme is responsible for rupture of the plaques and for the invasion and metastasis of a large number of cancers. The ability of ultrasonic excitation to induce thermal and mechanical effects has been used to release drugs from different carriers. However, majority of these studies were performed with low frequency ultrasound (LFUS) at kHz frequencies. Clinical usage of LFUS excitations will be limited due to harmful biological effects. Herein, we report our results on the release of encapsulated contents from substrate lipopeptide incorporated echogenic liposomes triggered by recombinant human MMP-9. The contents release was further enhanced by the application of diagnostic frequency (3 MHz) ultrasound. The echogenic liposomes were successfully imaged employing a medical ultrasound transducer (4 – 15 MHz). The conditioned cell culture media from cancer cells (secreting MMP-9) released the encapsulated dye from the liposomes (30 – 50%) and this release is also increased (50 – 80%) by applying diagnostic frequency ultrasound (3 MHz) for 3 minutes. With further developments, these liposomes have the potential to serve as multimodal carriers for triggered release and simultaneous ultrasound imaging.
Details
- Title: Subtitle
- Ultrasound Enhanced Matrix Metalloproteinase-9 Triggered Release of Contents from Echogenic Liposomes
- Creators
- Rahul Nahire - North Dakota State UniversityShirshendu Paul - University of DelawareMichael D. Scott - North Dakota State UniversityRaushan K. Singh - North Dakota State UniversityWallace W. Muhonen - University of North DakotaJohn Shabb - University of North DakotaKara N. Gange - North Dakota State UniversityD. K. Srivastava - North Dakota State UniversityKausik Sarkar - George Washington UniversitySanku Mallik - North Dakota State University
- Resource Type
- Journal article
- Publication Details
- Molecular pharmaceutics, Vol.9(9), pp.2554-2564
- DOI
- 10.1021/mp300165s
- PMID
- 22849291
- PMCID
- PMC3433587
- ISSN
- 1543-8384
- eISSN
- 1543-8392
- Language
- English
- Date published
- 08/15/2012
- Academic Unit
- Orthopedics and Rehabilitation
- Record Identifier
- 9984304714802771
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