Ultrasound-Mediated Microbubble Destruction Suppresses Melanoma Tumor Growth

Kee W Jang; Dongrim Seol; Lei Ding; Tae-Hong Lim; Joseph A Frank; James A Martin

doi:10.1016/j.ultrasmedbio.2017.12.011

Back

Ultrasound-Mediated Microbubble Destruction Suppresses Melanoma Tumor Growth

Journal article

Open access

Peer reviewed

Ultrasound-Mediated Microbubble Destruction Suppresses Melanoma Tumor Growth

Kee W Jang, Dongrim Seol, Lei Ding, Tae-Hong Lim, Joseph A Frank and James A Martin

Ultrasound in medicine & biology, Vol.44(4), pp.831-839

04/2018

DOI: 10.1016/j.ultrasmedbio.2017.12.011

PMCID: PMC5826859

PMID: 29361373

Files and links (1)

url

https://doi.org/10.1016/j.ultrasmedbio.2017.12.011View

Published (Version of record) Open Access

Abstract

Melanoma is one of the most aggressive types of cancer, and its incidence has increased rapidly in the past few decades. In this study, we investigated a novel treatment approach, the use of low-intensity ultrasound (2.3 W/cm at 1 MHz)-mediated Optison microbubble (MB) destruction (UMMD) to treat melanoma in a flank tumor model. The effect of UMMD was first evaluated in the melanoma cell line B16 F10 (B16) in vitro and then in mice inoculated with B16 cells. MB B16 cells were exposed to US in vitro, resulting in significant cell death proportional to duty cycle (R = 0.74): approximately 30%, 50%, 80% and 80% cell death at 10%, 30%, 50% and 100% DC respectively. Direct implantation of tumors with MBs, followed by sonication, resulted in retarded tumor growth and improved survival (p = 0.0106). Immunohistochemical analyses confirmed the significant changes in expression of the cell proliferation marker Ki67 (p = 0.037) and a microtubule-associated protein 2 (p = 0.048) after US + MB treatment. These results suggest that UMMD could be used as a possible treatment approach in isolated melanoma and has the potential to translate to clinical trials.

Contrast Media - pharmacology

Albumins - pharmacology

Animals

Mice, Inbred C57BL

Cell Death

Microbubbles

Ultrasonics - methods

Female

Fluorocarbons - pharmacology

Mice

Melanoma - therapy

Disease Models, Animal

Details

Title: Subtitle: Ultrasound-Mediated Microbubble Destruction Suppresses Melanoma Tumor Growth
Creators: Kee W Jang - Frank Laboratory, Radiology and Imaging Sciences, Clinical Center, National Institutes of Health, Bethesda, Maryland, USA. Electronic address: kee.jang@nih.gov
Dongrim Seol - Department of Orthopaedics and Rehabilitation, The University of Iowa, Iowa City, Iowa, USA
Lei Ding - Jiangnan University Wuxi Medical School, Wuxi, Jiangsu, China
Tae-Hong Lim - Department of Biomedical Engineering, The University of Iowa, Iowa City, Iowa, USA
Joseph A Frank - Frank Laboratory, Radiology and Imaging Sciences, Clinical Center, National Institutes of Health, Bethesda, Maryland, USA; National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, Bethesda, Maryland, USA
James A Martin - Department of Orthopaedics and Rehabilitation, The University of Iowa, Iowa City, Iowa, USA
Resource Type: Journal article
Publication Details: Ultrasound in medicine & biology, Vol.44(4), pp.831-839
Publisher: England
DOI: 10.1016/j.ultrasmedbio.2017.12.011
PMID: 29361373
PMCID: PMC5826859
ISSN: 0301-5629
eISSN: 1879-291X
Grant note: name: Department of Orthopaedics and Rehabilitation and Pharmaceutics and Translation Therapeutics at the University of Iowa; name: the Intramural Research Program of the National Institutes of Health Clinical Center; DOI: 10.13039/100000070, name: the National Institute of Biomedical Imaging and Bioengineering
Language: English
Date published: 04/2018
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Orthodontics; Pharmaceutical Sciences and Experimental Therapeutics; Orthopedics and Rehabilitation
Record Identifier: 9984040398702771

Metrics

26 Record Views

15 Times Cited - Web of Science