Journal article
Ultrasound stimulation restores impaired neovascularization-related capacities of human circulating angiogenic cells
Cardiovascular research, Vol.95(4), pp.448-459
09/01/2012
DOI: 10.1093/cvr/cvs173
PMID: 22641844
Abstract
Unsatisfactory effects of therapeutic angiogenesis in critical limb ischaemia may be ascribed to use of circulating angiogenic cells (CACs) derived from atherosclerotic patients with impaired neovascularization-related capacities. We tested whether ultrasound cell stimulation can restore the impaired capacities.
During culture of human peripheral blood-derived mononuclear cells for 4days to achieve CACs, we stimulated the cells in culture daily with low-intensity pulsed ultrasound stimulation (LIPUS). Application of LIPUS to cells in culture derived from healthy volunteers augmented the generation and migration capacities of CACs, increased concentrations of angiopoietin 2 and nitrogen oxides in the culture medium, and increased the expression of phosphorylated-Akt and endothelial nitric oxide synthase in CACs on western blotting. Application of LIPUS to cells in culture derived from atherosclerotic patients also augmented the generation and migration capacities of CACs. Although neovascularization in the ischaemic hindlimb of athymic nude mice was impaired after intramuscular injection of CACs derived from atherosclerotic patients compared with that using CACs derived from healthy volunteers, LIPUS of the cells in culture derived from atherosclerotic patients restored the neovascularization capacities.
Therapeutic angiogenesis with LIPUS-pre-treated CACs may be a new strategy to rescue critical limb ischaemia in atherosclerotic patients.
Details
- Title: Subtitle
- Ultrasound stimulation restores impaired neovascularization-related capacities of human circulating angiogenic cells
- Creators
- Yasuyuki Toyama - Kurume UniversityKen-ichiro Sasaki - Kurume UniversityKatsuro Tachibana - Fukuoka UniversityTakafumi Ueno - Kurume UniversityHidemi Kajimoto - Kurume UniversityShinji Yokoyama - Kurume UniversityMasanori Ohtsuka - Kurume UniversityHiroshi Koiwaya - Kurume UniversityTakaharu Nakayoshi - Kurume UniversityYoshiaki Mitsutake - Kurume UniversityHidetoshi Chibana - Kurume UniversityNaoki Itaya - Kurume UniversityTsutomu Imaizumi - Kurume University
- Resource Type
- Journal article
- Publication Details
- Cardiovascular research, Vol.95(4), pp.448-459
- DOI
- 10.1093/cvr/cvs173
- PMID
- 22641844
- NLM abbreviation
- Cardiovasc Res
- ISSN
- 0008-6363
- eISSN
- 1755-3245
- Publisher
- Oxford Univ Press
- Number of pages
- 12
- Grant note
- Strategic Research Foundation for Private Universities from MEXT, Japan Japan Cardiovascular Research Foundation 22300182 / Grants-in-Aid for Scientific Research; Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT); Japan Society for the Promotion of Science; Grants-in-Aid for Scientific Research (KAKENHI)
- Language
- English
- Date published
- 09/01/2012
- Academic Unit
- Cardiology; Stead Family Department of Pediatrics
- Record Identifier
- 9984961113802771
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