Ultrastructural Localization of the Herpes Simplex Virus Type 1 UL31, UL34, and US3 Proteins Suggests Specific Roles in Primary Envelopment and Egress of Nucleocapsids

Ashley E Reynolds; Elizabeth G Wills; Richard J Roller; Brent J Ryckman; Joel D Baines

doi:10.1128/JVI.76.17.8939-8952.2002

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Ultrastructural Localization of the Herpes Simplex Virus Type 1 UL31, UL34, and US3 Proteins Suggests Specific Roles in Primary Envelopment and Egress of Nucleocapsids

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Ultrastructural Localization of the Herpes Simplex Virus Type 1 UL31, UL34, and US3 Proteins Suggests Specific Roles in Primary Envelopment and Egress of Nucleocapsids

Ashley E Reynolds, Elizabeth G Wills, Richard J Roller, Brent J Ryckman and Joel D Baines

Journal of virology, Vol.76(17), pp.8939-8952

09/2002

DOI: 10.1128/JVI.76.17.8939-8952.2002

PMCID: PMC136992

PMID: 12163613

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Abstract

The wild-type U L 31, U L 34, and U S 3 proteins localized on nuclear membranes and perinuclear virions; the U S 3 protein was also on cytoplasmic membranes and extranuclear virions. The U L 31 and U L 34 proteins were not detected in extracellular virions. U S 3 deletion caused (i) virion accumulation in nuclear membrane invaginations, (ii) delayed virus production onset, and (iii) reduced peak virus titers. These data support the herpes simplex virus type 1 deenvelopment-reenvelopment model of virion egress and suggest that the U S 3 protein plays an important, but nonessential, role in the egress pathway.

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Title: Subtitle: Ultrastructural Localization of the Herpes Simplex Virus Type 1 UL31, UL34, and US3 Proteins Suggests Specific Roles in Primary Envelopment and Egress of Nucleocapsids
Creators: Ashley E Reynolds - Department of Microbiology, University of Iowa, Iowa City, Iowa 52242
Elizabeth G Wills - Department of Microbiology, University of Iowa, Iowa City, Iowa 52242
Richard J Roller - Department of Microbiology, University of Iowa, Iowa City, Iowa 52242
Brent J Ryckman - Department of Microbiology, University of Iowa, Iowa City, Iowa 52242
Joel D Baines - Department of Microbiology, University of Iowa, Iowa City, Iowa 52242
Resource Type: Journal article
Publication Details: Journal of virology, Vol.76(17), pp.8939-8952
DOI: 10.1128/JVI.76.17.8939-8952.2002
PMID: 12163613
PMCID: PMC136992
NLM abbreviation: J Virol
ISSN: 0022-538X
eISSN: 1098-5514
Publisher: American Society for Microbiology
Language: English
Date published: 09/2002
Academic Unit: Microbiology and Immunology
Record Identifier: 9984002388102771

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