Journal article
Ultrastructural localization of calsequestrin in rat skeletal muscle by immunoferritin labeling of ultrathin frozen sections
The Journal of cell biology, Vol.97(5), pp.1573-1581
1983
DOI: 10.1083/jcb.97.5.1573
PMID: 6355123
Abstract
The ultrastructural localization of calsequestrin in rat skeletal muscle (gracilis) was determined by indirect immunoferritin labeling of ultrathin frozen sections. Calsequestrin was found in the lumen of transversely and longitudinally oriented terminal cisternae but was absent from most of the longitudinal sarcotubules and the fenestrated sarcoplasmic reticulum. Calsequestrin was occasionally observed in vesicular structures found in the central region of the I band. Since calsequestrin is believed to provide the major site of Ca2+ sequestration in the sarcoplasmic reticulum, the present results support the view that Ca2+, transported to the lumen of the sarcoplasmic reticulum, is preferentially sequestered in the terminal cisternae, but they also suggest that additional Ca2+ sequestration may occur near the center of the I band.
Details
- Title: Subtitle
- Ultrastructural localization of calsequestrin in rat skeletal muscle by immunoferritin labeling of ultrathin frozen sections
- Creators
- A. O JORGENSEN - Univ. Toronto, dep. anatomyA. C.-Y SHEN - Univ. Toronto, dep. anatomyK. P CAMPBELL - Univ. Toronto, dep. anatomyD. H MACLENNAN - Univ. Toronto, dep. anatomy
- Resource Type
- Journal article
- Publication Details
- The Journal of cell biology, Vol.97(5), pp.1573-1581
- Publisher
- Rockefeller University Press; New York, NY
- DOI
- 10.1083/jcb.97.5.1573
- PMID
- 6355123
- ISSN
- 0021-9525
- eISSN
- 1540-8140
- Language
- English
- Date published
- 1983
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute
- Record Identifier
- 9984068265002771
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