Journal article
Ultrastructural localization of the cytoplasmic distribution of gamma tubulin in induced differentiating human leukemia cells
Journal of histotechnology, Vol.30(1), pp.35-40
03/01/2007
DOI: 10.1179/his.2007.30.1.35
Abstract
gamma-Tubulin is an essential component of the microtubule nucleation-organizing center (MTOC) and plays a crucial role during mitosis and differentiation. Because the gamma-tubulin content of individual cells is very low, we relied principally on the high resolution and sensitivity afforded by immunoelectron microscopy and used polyclonal antibody against gamma-tubulin in undifferentiated and induced differentiated human promyelocytic leukemia cells (HL-60) by 1 mu M all-trans retinoic acid. The effectiveness of this approach was demonstrated by the distribution of gamma-tubulin from differentiated HL-60 cells relative to undifferentiated cells. Our studies revealed that, like the situation in undifferentiated cells, gamma-tubulin labeling is mostly found in the pericentriolar region, with a few instances in the cytoplasm. However, in the differentiated cells, serial immuno-gold analysis indicated that the amount of gamma-tubulin in noncentrosomal region of cytoplasm was almost greater than three times the amount of gamma-tubulin in undifferentiated cells.
Details
- Title: Subtitle
- Ultrastructural localization of the cytoplasmic distribution of gamma tubulin in induced differentiating human leukemia cells
- Creators
- Shahin Ahmadian - Univ Tehran, Inst Biochem & Biophys, Dept Biochem, Tehran, IranAhmad Shariftabrizi - University of TehranReza Emadi - Univ Tehran, Inst Biochem & Biophys, Dept Biochem, Tehran, Iran
- Resource Type
- Journal article
- Publication Details
- Journal of histotechnology, Vol.30(1), pp.35-40
- Publisher
- Taylor & Francis
- DOI
- 10.1179/his.2007.30.1.35
- ISSN
- 0147-8885
- eISSN
- 2046-0236
- Number of pages
- 6
- Grant note
- 521/3/582 / Research Council of the University of Tehran; University of Tehran
- Language
- English
- Date published
- 03/01/2007
- Academic Unit
- Radiology
- Record Identifier
- 9984318799302771
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