Journal article
Ultrastructural, physiological and proteomic analysis of Nostoc flagelliforme in response to dehydration and rehydration
Journal of proteomics, Vol.75(18), pp.5604-5627
10/22/2012
DOI: 10.1016/j.jprot.2012.07.041
Abstract
Nostoc flagelliforme must undergo a dehydration/rehydration cycle during its growth stages; the mechanisms underlying this constraint are examined. The novel insights into N. flagelliforme's response to desiccation and rehydration at ultrastructural, physiological and proteomic levels were offered. The structure of colonies and cells remained unchanged in response to dehydration and rehydration treatments except that the sheath appeared shrunken, and both the quantity and volume of vacuoles were decreased when dehydrated compared with rehydration. A significant increase in photosynthesis, respiration, total Rubisco activity, superoxide anion level, SOD, CAT, POD, nitrogenase and glutamine synthetase (GS) activities in response to rehydration was noted, whereas H2O2, ammonium, proline and glutamate contents all registered a decrease. 32 differentially expressed proteins between dehydrated and rehydrated colonies were categorized according to their predicted functions into secretion, signaling, transcription and translation, antioxidative processes, nitrogen metabolism, energy metabolism, lipid metabolism and chaperonin. The dehydration is a quiescent state in which metabolism is down-regulated, upon rehydration, a metabolic shift occurs from quiescent to active. The specific metabolic and regulated mechanisms to accommodate the dehydration/rehydration cycle in N. flagelliforme is reported here.
Morphological changes of N. flagelliforme upon dehydration and rehydration. [Display omitted]
► Cell ultrastructure maintains stability in N. flagelliforme under water deficiency.
► Specific metabolic mechanism is related to the adaptation to xeric conditions.
► Some proteins may serve as molecular markers in response to dehydration.
► Carbohydrate and energy metabolism and antioxidation are important in rehydration.
► ROS detoxification pathway was proposed to cope with dehydration/rehydration cycle.
Details
- Title: Subtitle
- Ultrastructural, physiological and proteomic analysis of Nostoc flagelliforme in response to dehydration and rehydration
- Creators
- Wenyu Liang - Fujian Agriculture and Forestry UniversityYouwen Zhou - School of Life Sciences, Ningxia University, Yinchuan 750021, PR ChinaLingxia Wang - Fujian Agriculture and Forestry UniversityXiangrong You - Fujian Agriculture and Forestry UniversityYaping Zhang - Ningxia UniversityChi-Lien Cheng - University of Iowa, BiologyWei Chen - Fujian Agriculture and Forestry University
- Resource Type
- Journal article
- Publication Details
- Journal of proteomics, Vol.75(18), pp.5604-5627
- Publisher
- Elsevier B.V
- DOI
- 10.1016/j.jprot.2012.07.041
- ISSN
- 1874-3919
- eISSN
- 1876-7737
- Grant note
- name: National Natural Science Grant of China, award: 31060038, 30760022
- Language
- English
- Date published
- 10/22/2012
- Academic Unit
- Biology
- Record Identifier
- 9983992075402771
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