Umbilical cord blood screening for cytomegalovirus DNA by quantitative PCR

Regan N Theiler; Angela M Caliendo; Sabine Pargman; B Denise Raynor; Sarah Berga; Melissa McPheeters; Denise J Jamieson

doi:10.1016/j.jcv.2006.08.019

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Umbilical cord blood screening for cytomegalovirus DNA by quantitative PCR

Journal article

Peer reviewed

Umbilical cord blood screening for cytomegalovirus DNA by quantitative PCR

Regan N Theiler, Angela M Caliendo, Sabine Pargman, B Denise Raynor, Sarah Berga, Melissa McPheeters and Denise J Jamieson

Journal of clinical virology, Vol.37(4), pp.313-316

12/01/2006

DOI: 10.1016/j.jcv.2006.08.019

PMID: 17035082

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Abstract

Cytomegalovirus (CMV) infection, which is the most common congenitally transmitted infection, affects approximately 1% of neonates worldwide. Despite its prevalence, no convenient screening test for neonatal CMV infection has been implemented. The purpose of this pilot study was to evaluate the feasibility and yield of screening umbilical cord blood for CMV DNA emiaby quantitative PCR. Umbilical cord blood was tested for CMV DNAemia using a commercial quantitative PCR assay. Maternal CMV serostatus at the time of delivery was assessed by testing for CMV IgG and IgM antibodies in serum. Screening for congenital CMV infection with PCR is easily incorporated into routine labor and delivery care using discarded cord blood specimens to identify neonates whose infection is otherwise undiagnosed. Among 433 infants tested, two (0.5%) had DNAemia detected in cord blood, one of whom was symptomatic, and both of whose mothers were CMV IgG positive and IgM negative. Viremic neonates identified by screening with PCR may be at high risk of developing long-term neurological complications of CMV infection and cannot reliably be identified using clinical presentation or maternal serology. Because of its convenience, cord blood CMV screening with PCR should be further investigated for incorporation into neonatal screening protocols.

Antibodies, Viral - analysis

Antibodies, Viral - blood

Cytomegalovirus - genetics

Cytomegalovirus - isolation & purification

Cytomegalovirus Infections - prevention & control

Cytomegalovirus Infections - transmission

DNA, Viral - blood

Female

Fetal Blood - virology

Humans

Infant, Newborn

Mass Screening - methods

Mass Screening - standards

Polymerase Chain Reaction

Prenatal Diagnosis - methods

Serologic Tests - standards

Details

Title: Subtitle: Umbilical cord blood screening for cytomegalovirus DNA by quantitative PCR
Creators: Regan N Theiler - Emory University
Angela M Caliendo - Emory University
Sabine Pargman - Emory University
B Denise Raynor - Emory University
Sarah Berga - Emory University
Melissa McPheeters - Michigan Medicine
Denise J Jamieson - Emory University
Resource Type: Journal article
Publication Details: Journal of clinical virology, Vol.37(4), pp.313-316
DOI: 10.1016/j.jcv.2006.08.019
PMID: 17035082
ISSN: 1386-6532
eISSN: 1873-5967
Language: English
Date published: 12/01/2006
Academic Unit: Obstetrics and Gynecology; VPMA - Administration
Record Identifier: 9984446548302771

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19 Times Cited - Web of Science