Understanding the progression of atherosclerosis through gene profiling and co-expression network analysis in Apob(tm2Sgy)Ldlr(tm1Her) double knockout mice

Vrushali Deshpande; Ankit Sharma; Rupak Mukhopadhyay; Lakshmi Narasimha Rao Thota; Madankumar Ghatge; Rajani Kanth Vangala; Vijay V Kakkar; Lakshmi Mundkur

doi:10.1016/j.ygeno.2016.04.007

Back

Understanding the progression of atherosclerosis through gene profiling and co-expression network analysis in Apob(tm2Sgy)Ldlr(tm1Her) double knockout mice

Journal article

Open access

Peer reviewed

Understanding the progression of atherosclerosis through gene profiling and co-expression network analysis in Apob(tm2Sgy)Ldlr(tm1Her) double knockout mice

Vrushali Deshpande, Ankit Sharma, Rupak Mukhopadhyay, Lakshmi Narasimha Rao Thota, Madankumar Ghatge, Rajani Kanth Vangala, Vijay V Kakkar and Lakshmi Mundkur

Genomics (San Diego, Calif.), Vol.107(6), pp.239-247

06/2016

DOI: 10.1016/j.ygeno.2016.04.007

PMID: 27133569

Files and links (1)

url

https://doi.org/10.1016/j.ygeno.2016.04.007View

Published (Version of record) Open Access

Abstract

The objective of the study was to gain molecular insights into the progression of atherosclerosis in Apob(tm2Sgy)Ldlr(tm1Her) mice, using transcriptome profiles. Weighted gene co network analysis (WGCNA) and time course analysis using limma were used to study disease progression from 0 to 20weeks. Five co-expression modules were identified by WGCNA using the expression values of 2153 genes. Genes associated with autophagy, endoplasmic reticulum stress, inflammation and lipid metabolism were differentially expressed at early stages of atherosclerosis. Time course analysis highlighted activation of inflammatory gene signaling at 4weeks, cell proliferation and calcification at 8weeks, amyloid like structures and oxidative stress at 14weeks and enhanced production of inflammatory cytokines at 20weeks. Our results suggest that maximum gene perturbations occur during early atherosclerosis which could be the danger signals associated with subclinical disease. Understanding these genes and associated pathways can help in improvement of diagnostic and therapeutic targets for atherosclerosis.

Animals

Apolipoproteins B - genetics

Atherosclerosis - genetics

Atherosclerosis - pathology

Autophagy - genetics

Disease Models, Animal

Disease Progression

Endoplasmic Reticulum Stress - genetics

Gene Expression Regulation

Gene Regulatory Networks

Humans

Inflammation - genetics

Inflammation - pathology

Lipid Metabolism - genetics

Mice

Mice, Knockout

Oxidative Stress - genetics

Receptors, LDL - drug effects

Details

Title: Subtitle: Understanding the progression of atherosclerosis through gene profiling and co-expression network analysis in Apob(tm2Sgy)Ldlr(tm1Her) double knockout mice
Creators: Vrushali Deshpande - Thrombosis Research Institute
Ankit Sharma - Manipal Academy of Higher Education
Rupak Mukhopadhyay - Tezpur University
Lakshmi Narasimha Rao Thota - Thrombosis Research Institute
Madankumar Ghatge - Thrombosis Research Institute
Rajani Kanth Vangala - Thrombosis Research Institute
Vijay V Kakkar - Thrombosis Research Institute
Lakshmi Mundkur - Thrombosis Research Institute
Resource Type: Journal article
Publication Details: Genomics (San Diego, Calif.), Vol.107(6), pp.239-247
DOI: 10.1016/j.ygeno.2016.04.007
PMID: 27133569
NLM abbreviation: Genomics
ISSN: 0888-7543
eISSN: 1089-8646
Language: English
Date published: 06/2016
Academic Unit: Internal Medicine
Record Identifier: 9984949185602771

Metrics

4 Record Views