Unique Antimicrobial Effects of Platelet-Rich Plasma and Its Efficacy as a Prophylaxis to Prevent Implant-Associated Spinal Infection

Hongshuai Li; Therwa Hamza; John E Tidwell; Nina Clovis; Bingyun Li

doi:10.1002/adhm.201200465

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Journal article

Unique Antimicrobial Effects of Platelet-Rich Plasma and Its Efficacy as a Prophylaxis to Prevent Implant-Associated Spinal Infection

Hongshuai Li, Therwa Hamza, John E Tidwell, Nina Clovis and Bingyun Li

Advanced healthcare materials, Vol.2(9), pp.1277-1284

09/2013

DOI: 10.1002/adhm.201200465

PMCID: PMC3774283

PMID: 23447088

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Abstract

Platelet-rich-plasma (PRP) has attracted great attention and has been increasingly used for a variety of clinical applications including orthopaedic surgeries, periodontal and oral surgeries, maxillofacial surgeries, plastic surgeries, and sports medicine. However, very little is known about the antimicrobial activities of PRP. In this study, PRP is found to have antimicrobial properties both in vitro and in vivo. In vitro , the antimicrobial properties of PRP have been found to be bacterial strain specific and time specific: PRP has significantly (80–100 fold reduction in colony forming units) inhibited the growth of methicillin sensitive and methicillin resistant Staphylococcus aureus , Group A streptococcus, and Neisseria gonorrhoeae within the first few hours but it has no significant antimicrobial properties against E. coli and Pseudomonas . The antimicrobial properties of PRP also depend on the concentration of thrombin. In vivo , an implant-associated spinal infection rabbit model has been established and used to evaluate the antimicrobial and wound healing properties of PRP. Compared to the infection controls, PRP treatment has resulted in significant reduction in bacterial colonies in bone samples at all time points studied (i.e. 1, 2, and 3 weeks) and significant increase in mineralized tissues (thereby better bone healing) at post-operative weeks 2 and 3. PRP therefore may be a useful adjunct strategy against post-operative implant-associated infections.

implant infection

antimicrobial

platelet-rich plasma

rabbit spine infection model

Staphylococcus aureus

Details

Title: Subtitle: Unique Antimicrobial Effects of Platelet-Rich Plasma and Its Efficacy as a Prophylaxis to Prevent Implant-Associated Spinal Infection
Creators: Hongshuai Li - Department of Orthopaedics, School of Medicine, West Virginia University, Morgantown, WV 26506, USA. Department of Orthopaedics, University of Pittsburgh, Pittsburgh, Pennsylvania 15219, USA
Therwa Hamza - Department of Orthopaedics, School of Medicine, West Virginia University, Morgantown, WV 26506, USA
John E Tidwell - Department of Orthopaedics, School of Medicine, West Virginia University, Morgantown, WV 26506, USA
Nina Clovis - Department of Orthopaedics, School of Medicine, West Virginia University, Morgantown, WV 26506, USA
Bingyun Li - Department of Orthopaedics, School of Medicine, West Virginia University, Morgantown, WV 26506, USA. WVNano Initiative, Morgantown, WV 26506, USA. Mary Babb Randolph Cancer Center, Morgantown, WV 26506, USA
Resource Type: Journal article
Publication Details: Advanced healthcare materials, Vol.2(9), pp.1277-1284
DOI: 10.1002/adhm.201200465
PMID: 23447088
PMCID: PMC3774283
NLM abbreviation: Adv Healthc Mater
ISSN: 2192-2640
eISSN: 2192-2659
Grant note: P20 RR016440 || RR / National Center for Research Resources : NCRR
Language: English
Date published: 09/2013
Academic Unit: Orthopedics and Rehabilitation; Radiation Oncology
Record Identifier: 9984106393702771

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