Unique and redundant β-catenin regulatory roles of two Dishevelled paralogs during C. elegans asymmetric cell division

Austin T Baldwin; Amy M Clemons; Bryan T Phillips

doi:10.1242/jcs.175802

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Unique and redundant β-catenin regulatory roles of two Dishevelled paralogs during C. elegans asymmetric cell division

Journal article

Open access

Peer reviewed

Unique and redundant β-catenin regulatory roles of two Dishevelled paralogs during C. elegans asymmetric cell division

Austin T Baldwin, Amy M Clemons and Bryan T Phillips

Journal of cell science, Vol.129(5), pp.983-993

03/01/2016

DOI: 10.1242/jcs.175802

PMCID: PMC6518166

PMID: 26795562

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Abstract

The Wnt/β-catenin signaling pathway is utilized across metazoans. However, the mechanism of signal transduction, especially dissociation of the β-catenin destruction complex by Dishevelled proteins, remains controversial. Here, we describe the function of the Dishevelled paralogs DSH-2 and MIG-5 in the Wnt/β-catenin asymmetry (WβA) pathway in Caenorhabditis elegans, where WβA drives asymmetric cell divisions throughout development. We find that DSH-2 and MIG-5 redundantly regulate cell fate in hypodermal seam cells. Similarly, both DSH-2 and MIG-5 are required for positive regulation of SYS-1 (a C. elegans β-catenin), but MIG-5 has a stronger effect on the polarity of SYS-1 localization. We show that MIG-5 controls cortical APR-1 (the C. elegans APC) localization. DSH-2 and MIG-5 both regulate the localization of WRM-1 (another C. elegans β-catenin), acting together as negative regulators of WRM-1 nuclear localization. Finally, we demonstrate that overexpression of DSH-2 or MIG-5 in seam cells leads to stabilization of SYS-1 in the anterior seam daughter, solidifying the Dishevelled proteins as positive regulators of SYS-1. Overall, we have further defined the role of Dishevelled in the WβA signaling pathway, and demonstrated that DSH-2 and MIG-5 regulate cell fate, β-catenin nuclear levels and the polarity of β-catenin regulation.

Animals

Asymmetric Cell Division

beta Catenin - metabolism

Caenorhabditis elegans - cytology

Caenorhabditis elegans - metabolism

Caenorhabditis elegans Proteins - metabolism

Caenorhabditis elegans Proteins - physiology

Cell Cycle Proteins - physiology

Cell Differentiation

Cytoskeletal Proteins - metabolism

Dishevelled Proteins - physiology

Protein Stability

Protein Transport

Transcription Factors - metabolism

Wnt Signaling Pathway

Details

Title: Subtitle: Unique and redundant β-catenin regulatory roles of two Dishevelled paralogs during C. elegans asymmetric cell division
Creators: Austin T Baldwin - Department of Biology, University of Iowa, 143 Biology Building, Iowa City, IA 52242-1324, USA
Amy M Clemons - Department of Biology, University of Iowa, 143 Biology Building, Iowa City, IA 52242-1324, USA
Bryan T Phillips - Department of Biology, University of Iowa, 143 Biology Building, Iowa City, IA 52242-1324, USA bryan-phillips@uiowa.edu
Resource Type: Journal article
Publication Details: Journal of cell science, Vol.129(5), pp.983-993
DOI: 10.1242/jcs.175802
PMID: 26795562
PMCID: PMC6518166
NLM abbreviation: J Cell Sci
ISSN: 0021-9533
eISSN: 1477-9137
Grant note: P40OD01440 / NIH HHS P30 CA086862 / NCI NIH HHS R01 GM114007 / NIGMS NIH HHS
Language: English
Date published: 03/01/2016
Academic Unit: Biology
Record Identifier: 9984217530102771

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