Journal article
Unveiling structure-activity relationships of proanthocyanidins with dentin collagen
Dental materials, Vol.37(11), pp.1633-1644
11/01/2021
DOI: 10.1016/j.dental.2021.08.013
PMCID: PMC8791559
PMID: 34563363
Abstract
Objective. To elucidate the structure-activity relationships (SARs) of proanthocyanidins (PACs) with type I collagen using sixteen chemically defined PACs with degree of polymerization (DP) 2-6. Methods. Under a dentin model, the biomimicry of PACs with type I collagen was investigated by dynamic mechanical analysis (DMA) and infrared spectroscopy. The dentin matrix was modified with PACs from Pinus massoniana [monomers (Mon-1 and Mon-2), dimers (Dim-1 Dim-4), trimers (Tri-1-Tri-4), tetramers (Tet-1-Tet-5), and hexamer (Hex-1)]. A strain sweep method in a 3-point bending submersion clamp was used to assess the viscoelastic properties [storage (E'), loss (E"), and complex moduli (E*) and tan 8] of the dentin matrix before and after biomodification. Biochemical analysis of the dentin matrix was assessed with FTIR spectroscopy. Data were statistically analyzed using one-way ANOVA and post-hoc tests ( = 0.05). Results. DP had a significant effect on modified dentin moduli (tetramers approximate to trimers > hexamers approximate to dimers > monomers approximate to control, p < 0.001). Trimers and tetramers yielded 6-to 8-fold increase in the mechanical properties of modified dentin and induced conformational changes to the secondary structure of collagen. Modifications to the tertiary structure of collagen was shown in all PAC modified-dentin matrices. Significance. Findings establish three key SARs: (i) increasing DP generally enhances biomimicry potential of PACs in modulating the mechanical and chemical properties of dentin (ii) the secondary structure of dentin collagen is affected by the position of B-type inter-flavanyl linkages (4,6 6 and 4,6 8); and (iii) the terminal monomeric flavan-3-ol unit plays a modulatory role in the viscoelasticity of dentin. (c) 2021 The Academy of Dental Materials. Published by Elsevier Inc. All rights reserved.
Details
- Title: Subtitle
- Unveiling structure-activity relationships of proanthocyanidins with dentin collagen
- Creators
- Mariana Reis - Marquette UniversityBin Zhou - University of Illinois ChicagoYvette Alania - University of Illinois ChicagoAriene A. Leme-Kraus - University of Illinois ChicagoShuxi Jing - Univ Illinois, Coll Pharm, Pharmacognosy Inst, Chicago, IL 60612 USAJames B. McAlpine - University of Illinois ChicagoShao-Nong Chen - University of Illinois ChicagoGuido F. Pauli - University of Illinois ChicagoAna K. Bedran-Russo - Marquette University
- Resource Type
- Journal article
- Publication Details
- Dental materials, Vol.37(11), pp.1633-1644
- DOI
- 10.1016/j.dental.2021.08.013
- PMID
- 34563363
- PMCID
- PMC8791559
- NLM abbreviation
- Dent Mater
- ISSN
- 0109-5641
- eISSN
- 1879-0097
- Publisher
- Elsevier
- Number of pages
- 12
- Language
- English
- Date published
- 11/01/2021
- Academic Unit
- Operative Dentistry
- Record Identifier
- 9984367749402771
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