Journal article
Up-regulation of p21 WAF1 expression in myeloid cells is activated by the protein kinase C pathway
British journal of cancer, Vol.76(12), pp.1554-1557
12/1997
DOI: 10.1038/bjc.1997.595
PMID: 9413940
Abstract
Phorbol-12-myristate-13-acetate (PMA) induces p21WAF-1 expression in human myeloid leukaemic HL-60 cells. We show that this induction is specifically mediated by protein kinase C (PKC). In addition, the PKC inhibitor Ro 31-8220 with predominant PKC-alpha isoform specificity almost completely inhibited PMA-induced up-regulation of p21WAF1 in HL-60 cells as well as in the myelomonocytic leukaemic U937 cells. Pretreatment of HL-60 cells with Ro 31-8220 also inhibited PMA-induced activation of c-raf-1, a known PKC alpha target. In the phorbol ester-tolerant HL-60 subline (PET) with PKC-beta isoform deficiency PMA or bryostatin-1 induced p21WAF1 expression, but to a lesser extent than in wild-type HL-60 cells. In PET cells, Ro 31-8220 also inhibited PMA and bryostatin-1-induced up-regulation of p21WAF1 expression. Our findings indicate that at least in HL-60 cells up-regulation of p21WAF-1 is specifically activated by PKC. We suggest that PKC isoforms other than beta, presumably the PKC-alpha isoform, are involved in this process.
Details
- Title: Subtitle
- Up-regulation of p21 WAF1 expression in myeloid cells is activated by the protein kinase C pathway
- Creators
- J Schwaller - Department of Clinical Research, University of BerneU.R Peters - Department of Clinical Research, University of Berne, Institute of Medical Oncology, University of BerneTh Pabst - Department of Clinical Research, University of BerneG Niklaus - Department of Clinical Research, University of BerneD.E Macfarlane - Department of Medicine, Veterans Administration on Hospital, University of IowaM.F Fey - Department of Clinical Research, University of Berne, Institute of Medical Oncology, University of BerneA Tobler - Department of Clinical Research, University of Berne, Central Haematology Laboratory, University of Berne, Central Haematology Laboratory, University of Berne, Inselspital
- Resource Type
- Journal article
- Publication Details
- British journal of cancer, Vol.76(12), pp.1554-1557
- DOI
- 10.1038/bjc.1997.595
- PMID
- 9413940
- NLM abbreviation
- Br J Cancer
- ISSN
- 0007-0920
- eISSN
- 1532-1827
- Language
- English
- Date published
- 12/1997
- Academic Unit
- Hematology, Oncology, and Blood & Marrow Transplantation; Internal Medicine
- Record Identifier
- 9984094709202771
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