Journal article
Uptake of Exogenous Gangliosides by Rat Brain Synaptosomes
Neurochemical research, Vol.23(12), pp.1515-1520
12/01/1998
DOI: 10.1023/A:1020971802722
PMID: 9821155
Abstract
Synaptosomes incorporated mixed brain gangliosides at a rapid initial rate followed by a slower phase of net movement from the protein-associated fraction into the membrane core. The pattern of incorporated gangliosides reflected the pattern available for incorporation. Intact synaptosomes incorporated ~100 pmol GM1/mg protein. Synaptosomes preincubated with proteolytic enzymes (trypsin, chymotrypsin, and papain) at different pH values (6.2, 7.4, 7.8) incorporated more exogenous gangliosides than synaptosomes preincubated in buffer alone. This effect was maximal at pH 7.8, though analysis of variance revealed that the proteolytic treatment and pH effects were probably independent processes. Overall uptake of exogenous gangliosides correlated significantly with amount of membrane protein loss, indicating that initial access of exogenous gangliosides to synaptosomal membranes is retarded by cell-surface proteins. These results suggest synaptosomes as a useful alternative to cultured cells for investigating the interaction of gangliosides with other cell surface constituents.[PUBLICATION ABSTRACT]
Details
- Title: Subtitle
- Uptake of Exogenous Gangliosides by Rat Brain Synaptosomes
- Creators
- Helen Young - The University of Texas at El PasoZoë Christian - The University of Texas at El PasoRafael Cabeza - The University of Texas at El PasoLouis Irwin - The University of Texas at El Paso
- Resource Type
- Journal article
- Publication Details
- Neurochemical research, Vol.23(12), pp.1515-1520
- Publisher
- Springer Nature B.V
- DOI
- 10.1023/A:1020971802722
- PMID
- 9821155
- ISSN
- 0364-3190
- eISSN
- 1573-6903
- Language
- English
- Date published
- 12/01/1998
- Academic Unit
- Molecular Physiology and Biophysics
- Record Identifier
- 9984695826802771
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