Use of In Vivo Imaging to Screen for Morphogenesis Phenotypes in Candida albicans Mutant Strains During Active Infection in a Mammalian Host

Rohan S Wakade; Damian J Krysan; Melanie Wellington

doi:10.3791/64258

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Use of In Vivo Imaging to Screen for Morphogenesis Phenotypes in Candida albicans Mutant Strains During Active Infection in a Mammalian Host

Journal article

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Use of In Vivo Imaging to Screen for Morphogenesis Phenotypes in Candida albicans Mutant Strains During Active Infection in a Mammalian Host

Rohan S Wakade, Damian J Krysan and Melanie Wellington

Journal of visualized experiments, Vol.188, e64258

10/12/2022

DOI: 10.3791/64258

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Abstract

Candida albicans is an important human pathogen. Its ability to switch between morphologic forms is central to its pathogenesis; these morphologic changes are regulated by a complex signaling network controlled in response to environmental stimuli. These regulatory components have been highly studied, but almost all studies use a variety of in vitro stimuli to trigger filamentation. To determine how morphogenesis is regulated during the pathogenesis process, we developed an in vivo microscopy system to obtain high spatial resolution images of organisms undergoing hyphal formation within the mammalian host. The protocol presented here describes the use of this system to screen small collections of C. albicans mutant strains, allowing us to identify key regulators of morphogenesis as it occurs at the site of infection. Representative results are presented, demonstrating that some regulators of morphogenesis, such as the transcriptional regulator Efg1, have consistent phenotypes in vitro and in vivo, whereas other regulators, such as adenyl cyclase (Cyr1), have significantly different phenotypes in vivo compared to in vitro

Details

Title: Subtitle: Use of In Vivo Imaging to Screen for Morphogenesis Phenotypes in Candida albicans Mutant Strains During Active Infection in a Mammalian Host
Creators: Rohan S Wakade - Roy J. and Lucille A. Carver College of Medicine
Damian J Krysan - Roy J. and Lucille A. Carver College of Medicine
Melanie Wellington - Roy J. and Lucille A. Carver College of Medicine
Resource Type: Journal article
Publication Details: Journal of visualized experiments, Vol.188, e64258
DOI: 10.3791/64258
eISSN: 1940-087X
Language: English
Date published: 10/12/2022
Academic Unit: Molecular Physiology and Biophysics; Microbiology and Immunology; Stead Family Department of Pediatrics; Infectious Disease (Pediatrics)
Record Identifier: 9984311460302771

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