Journal article
Use of a modified transwell culture to quantitate the contribution of non-IL-7 substances to growth of an IL-7-dependent Pre-B cell clone
Lymphokine and cytokine research, Vol.11(3), pp.167-173
1992
PMID: 1382627
Abstract
Clone 3 is a mouse pre-B cell line that cannot grow in standard tissue culture media but is immortalized by coculture with a bone marrow-derived feeder layer. In addition, clone 3 cells can be passaged indefinitely in recombinant interleukin-7 (IL-7) in the absence of a feeder layer if maintained at high cell density. Using monoclonal antibody to IL-7 and Transwells ligated to dialysis membranes, we have examined the relative contributions of IL-7, both endogenous and exogenous, and of "non-IL-7" feeder layer factors to growth promotion of clone 3 cells. There is synergy between feeder layers and exogenous IL-7 that is most marked when the latter is present in suboptimal concentrations. The synergizing activity is not neutralized by antibody to IL-7 and appears to be freely dialyzable. This "non-IL-7" effect is common to two different feeder layers, the one derived from bone marrow (3E) being an IL-7 producer, and the other, 3T3 fibroblasts, making no detectable IL-7. These experiments reveal a substantial contribution of the dialyzable moiety to the total feeder layer effect, and are the first to demonstrate cytokine-dependent low-molecular-weight synergy in a coculture. This demonstration is possible because the synergizing cofactor(s) can cross a semipermeable membrane whereas the cytokine and its neutralizing antibody cannot.
Details
- Title: Subtitle
- Use of a modified transwell culture to quantitate the contribution of non-IL-7 substances to growth of an IL-7-dependent Pre-B cell clone
- Creators
- M MUIRHEAD - Univ. Arkansas medical sci., veterans administration medical cent., Little Rock AR 72205, United StatesR. C SCHWARTZ - Univ. Arkansas medical sci., veterans administration medical cent., Little Rock AR 72205, United StatesT. J WALDSCHMIDT - Univ. Arkansas medical sci., veterans administration medical cent., Little Rock AR 72205, United States
- Resource Type
- Journal article
- Publication Details
- Lymphokine and cytokine research, Vol.11(3), pp.167-173
- Publisher
- Liebert; New York, NY
- PMID
- 1382627
- ISSN
- 1056-5477
- Language
- English
- Date published
- 1992
- Academic Unit
- Pathology
- Record Identifier
- 9984047696502771
Metrics
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