Journal article
Use of amphotropic retroviruses expressing human papillomavirus 16 E6 and E7 to determine the consequences of acute expression of the viral oncogenes and to establish immortalized human cell lines
Radiation oncology investigations, Vol.3(6), pp.315-319
1995
DOI: 10.1002/roi.2970030612
Abstract
We developed a series of amphotropic retroviruses expressing the E6 and E7 oncogenes from human papillomavirus (HPV) 16 and HPV 6. LXSN16E6E7 efficiently immortalized a wide variety of primary cell types, including epithelia from foreskin, cervix, breast, oral cavity, urogenital region, kidney, prostate, ovary, etc., as well as smooth muscle cells, endothelial cells, fibroblasts, hepatocytes, melanocytes, and others. Rare clones of keratinocytes were established by expression of E7 alone, and breast cells that had bypassed M0 were immortalized by E6 alone. However, in general, efficient immortalization required both E6 and E7 of HPV 16. The immortalized cell lines were nontumorigenic in nude mice and retained many of the differentiated characteristics of the primary cells; however, they invariably had chromosomal abnormalities.
Acute expression of E6 or E7 did not alter the doubling time of cells in culture; however, E7 disrupted normal suprabasal quiescence. No increase in aneuploidy or alteration in the rate of telomere shortening was observed before crisis, and cells continued to express markers of epithelial differentiation in organotypic culture. The ability of cells to bypass growth arrest signals was altered by the HPV oncogenes. © 1995 Wiley‐Liss, Inc.
Details
- Title: Subtitle
- Use of amphotropic retroviruses expressing human papillomavirus 16 E6 and E7 to determine the consequences of acute expression of the viral oncogenes and to establish immortalized human cell lines
- Creators
- D. A GallowayC. L HalbertG. W DemersS. A FosterR. A BlantonD MerrickA KlingelhutzJ. K McDougall
- Resource Type
- Journal article
- Publication Details
- Radiation oncology investigations, Vol.3(6), pp.315-319
- Publisher
- Wiley Subscription Services, Inc., A Wiley Company; New York
- DOI
- 10.1002/roi.2970030612
- ISSN
- 1065-7541
- eISSN
- 1520-6823
- Number of pages
- 5
- Grant note
- National Cancer Institute (CA4272; CA64795)
- Language
- English
- Date published
- 1995
- Academic Unit
- Microbiology and Immunology; Radiation Oncology
- Record Identifier
- 9984001163802771
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