Journal article
Use of benzyldimethyl- n-hexadecylammonium chloride (“16-BAC”), a cationic detergent, in an acidic polyacrylamide gel electrophoresis system to detect base labile protein methylation in intact cells
Analytical biochemistry, Vol.132(2), pp.231-235
1983
DOI: 10.1016/0003-2697(83)90001-5
PMID: 6625164
Abstract
A discontinuous polyacrylamide gel system operating at pH 4.0–1.5 which resolves proteins bearing base labile groups extracted from intact cells is described. It uses potassium phosphate buffer in the running and stacking gel and glycine as the trailing ion component. Proteins are solubilized with urea and benzyldimethyl-
n-hexadecylammonium chloride, a cationic detergent. The utility of the system is illustrated by fluorographs of the pattern of protein methylation in blood platelets and the HL60 promyelocyte cell line.
Details
- Title: Subtitle
- Use of benzyldimethyl- n-hexadecylammonium chloride (“16-BAC”), a cationic detergent, in an acidic polyacrylamide gel electrophoresis system to detect base labile protein methylation in intact cells
- Creators
- Donald E Macfarlane - University of Iowa Hospitals and Clinics, Department of Internal Medicine, Iowa City, Iowa 52242 USA
- Resource Type
- Journal article
- Publication Details
- Analytical biochemistry, Vol.132(2), pp.231-235
- Publisher
- Elsevier Inc
- DOI
- 10.1016/0003-2697(83)90001-5
- PMID
- 6625164
- ISSN
- 0003-2697
- eISSN
- 1096-0309
- Language
- English
- Date published
- 1983
- Academic Unit
- Hematology, Oncology, and Blood & Marrow Transplantation; Internal Medicine
- Record Identifier
- 9984094724302771
Metrics
10 Record Views