Use of collagen sponge incorporating transforming growth factor- β1 to promote bone repair in skull defects in rabbits

Hiroki Ueda; Liu Hong; Masaya Yamamoto; Keiji Shigeno; Masatoshi Inoue; Toshinari Toba; Makoto Yoshitani; Tatsuo Nakamura; Yasuhiko Tabata; Yasuhiko Shimizu

doi:10.1016/S0142-9612(01)00211-3

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Use of collagen sponge incorporating transforming growth factor- β1 to promote bone repair in skull defects in rabbits

Journal article

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Use of collagen sponge incorporating transforming growth factor- β1 to promote bone repair in skull defects in rabbits

Hiroki Ueda, Liu Hong, Masaya Yamamoto, Keiji Shigeno, Masatoshi Inoue, Toshinari Toba, Makoto Yoshitani, Tatsuo Nakamura, Yasuhiko Tabata and Yasuhiko Shimizu

Biomaterials, Vol.23(4), pp.1003-1010

2002

DOI: 10.1016/S0142-9612(01)00211-3

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Abstract

The objective of this study was to evaluate the potential of collagen sponge incorporating transforming growth factor- β1 (TGF- β1) to enhance bone repair. The collagen sponge was prepared by freeze-drying aqueous foamed collagen solution. Thermal cross-linking was performed in a vacuum at 140°C for periods ranging from 1 to 48 h to prepare a number of fine collagen sponges. When collagen sponges incorporating 125I-labeled TGF- β1 were placed in phosphate-buffered saline (PBS) solution at 37°C, a small amount of TGF- β1 was released for the first hour, but no further release was observed thereafter, irrespective of the amount of cross-linking time the sponges had received. Collagen sponges incorporating 125I-labeled TGF- β1 or simply labeled with 125I were implanted into the skin on the backs of mice. The radioactivity of the 125I-labeled TGF- β1 in the collagen sponges decreased with time; the amount of TGF- β1 remaining dependent on the cross-linking time. The in vivo retention of TGF- β1 was longer in those sponges that had been subjected to longer cross-linking times. The in vivo release profile of the TGF- β1 was matched with the degradation profile of the sponges. Scanning electron microscopic observation revealed no difference in structure among sponges subjected to different cross-linking times. The TGF- β1 immobilized in the sponges was probably released in vivo as a result of sponge biodegradation because TGF- β1 release did not occur in in vitro conditions in which sponges did not degrade. We applied collagen sponges incorporating 0.1 μg of TGF- β1 to skull defects in rabbits in stress-unloaded bone situations. Six weeks later, the skull defects were covered by newly formed bone, in marked contrast to the results obtained with a TGF- β1-free empty collagen sponge and 0.1 μg of free TGF- β1. We concluded that the collagen sponges were able to release biologically active TGF- β1 and were a promising material for bone repair.

Bone repair

Collagen sponge

Controlled release

In vivo degradation

TGF- β1

Details

Title: Subtitle: Use of collagen sponge incorporating transforming growth factor- β1 to promote bone repair in skull defects in rabbits
Creators: Hiroki Ueda - Kyoto University
Liu Hong - Kyoto University
Masaya Yamamoto - Kyoto University
Keiji Shigeno - Kyoto University
Masatoshi Inoue - Kyoto University
Toshinari Toba - Kyoto University
Makoto Yoshitani - Kyoto University
Tatsuo Nakamura - Kyoto University
Yasuhiko Tabata - Kyoto University
Yasuhiko Shimizu - Kyoto University
Resource Type: Journal article
Publication Details: Biomaterials, Vol.23(4), pp.1003-1010
DOI: 10.1016/S0142-9612(01)00211-3
ISSN: 0142-9612
eISSN: 1878-5905
Publisher: Elsevier Ltd
Language: English
Date published: 2002
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Prosthodontics; Craniofacial Anomalies Research Center; Dental Research
Record Identifier: 9984367629902771

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