Journal article
Use of molecular beacons to verify that the serine hydroxymethyltransferase pseudogene SHMT-ps1 is unique to the order Primates
Genome biology, Vol.2(2), research0006.1
01/01/2001
DOI: 10.1186/gb-2001-2-2-research0006
PMCID: PMC25776
PMID: 11182889
Abstract
Background: The serine hydroxymethyltransferase processed pseudogene SHMT-ps1 has been suggested to be unique to the order Primates because of the failure to amplify this sequence by PCR from genomic DNAs of any non-primate mammal species. Here, 'molecular beacon' probes specific to SHMT-ps1 were used in an attempt to verify this suggestion.
Results: In a search for SHMT-ps1-specific sequences using molecular beacons across a range of mammalian species, SHMT-ps1 was only found in primates. The molecular beacon assays also showed that SHMT-ps1 is present in both Old World and New World species but not among prosimians.
Conclusions: These results suggest that SHMT-ps1 originated close to the origin of the Anthropoidea, some 40 to 50 million years ago.
Details
- Title: Subtitle
- Use of molecular beacons to verify that the serine hydroxymethyltransferase pseudogene SHMT-ps1 is unique to the order Primates
- Creators
- Eric J. Devor - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Genome biology, Vol.2(2), research0006.1
- DOI
- 10.1186/gb-2001-2-2-research0006
- PMID
- 11182889
- PMCID
- PMC25776
- ISSN
- 1474-760X
- Language
- English
- Date published
- 01/01/2001
- Academic Unit
- Obstetrics and Gynecology
- Record Identifier
- 9983557408602771
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