Journal article
Use of peptide for selective and sensitive detection of an Anthrax biomarker via peptide recognition and surface‐enhanced Raman scattering
Journal of Raman Spectroscopy, Vol.41(2), pp.121-124
02/2010
DOI: 10.1002/jrs.2591
Abstract
A short 16‐amino acid peptide has been used in place of an antibody to selectively detect the specific Anthrax biomarker, protective antigen (PA), using surface‐enhanced Raman scattering (SERS). Peptides are more stable than antibodies under various biological conditions and are easily synthesized for a specific target. A peptide that has high affinity to PA was conjugated onto gold nanoparticles along with a Raman reporter and then incubated in various concentrations of PA. Parallel studies in which the peptide sequence was replaced with an antibody were performed to compare the performance of the two methodologies. Both the peptide and antibody functionalized nanoparticles were able to specifically detect PA concentrations down to 6.1 fM. These results demonstrate that these short, robust peptides can be used in the place of traditional antibodies to specifically recognize target biomarkers in the field for the potential diagnosis of disease. Copyright © 2010 John Wiley & Sons, Ltd. A short 16‐amino acid peptide has been used in place of an antibody to selectively detect the specific Anthrax biomarker, protective antigen (PA), down to 6.1 fM using surface‐enhanced Raman scattering (SERS).
Details
- Title: Subtitle
- Use of peptide for selective and sensitive detection of an Anthrax biomarker via peptide recognition and surface‐enhanced Raman scattering
- Creators
- Kyungtag RyuAmanda J HaesHye‐Yeon ParkSanghee NahJoungmok KimHoeil ChungMoon‐Young YoonSung‐Hwan Han
- Resource Type
- Journal article
- Publication Details
- Journal of Raman Spectroscopy, Vol.41(2), pp.121-124
- Publisher
- John Wiley & Sons, Ltd; Chichester, UK
- DOI
- 10.1002/jrs.2591
- ISSN
- 0377-0486
- eISSN
- 1097-4555
- Number of pages
- 4
- Language
- English
- Date published
- 02/2010
- Academic Unit
- Chemistry
- Record Identifier
- 9983985921602771
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