Journal article
Use of the alkaline comet assay for the detection of transplacental genotoxins in newborn mice
Mutation research. Genetic toxicology and environmental mutagenesis, Vol.653(1-2), pp.134-139
05/31/2008
DOI: 10.1016/j.mrgentox.2008.03.004
PMID: 18468946
Abstract
Several lines of evidence show that in utero exposure to different toxicants has greater consequences than their exposure during adult life. This may be due to involvement of critical developmental stages, physiological immaturity and the long later-life span over which disease may initiate, develop and progress. The in vivo alkaline comet (single-cell gel electrophoresis) assay has been favoured by the scientific community for the evaluation of genotoxins. The objective of this study was to demonstrate the suitability of alkaline comet assay in detecting transplacental genotoxins using newborn mice. Here, we report the successful use of the comet assay in detecting multi-organ genotoxicity of known transplacental genotoxins in newborn mice. Three well known transplacental genotoxic agents, cyclophosphamide (CP), mitomycin-C (MMC) and zidovudine (AZT) were tested in pregnant Swiss mice. These compounds were administered in the late gestational period (16-20th days of pregnancy) and the comet assay was performed with lymphocytes, bone marrow, liver and kidney cells of newborn mice. Significant DNA damage was observed in all the tissues with tested transplacental genotoxins. The results of the comet assay were confirmed by the micronucleus (MN) assay of the peripheral blood of newborn mice. The results of this study provide sufficient evidence that the comet assay can be applied successfully for the detection of transplacental genotoxins in newborn mice. (C) 2008 Elsevier B.V. All rights reserved.
Details
- Title: Subtitle
- Use of the alkaline comet assay for the detection of transplacental genotoxins in newborn mice
- Creators
- D. N. Tripathi - National Institute of Pharmaceutical Education and ResearchA. A. Pawar - National Institute of Pharmaceutical Education and ResearchA. Vikram - National Institute of Pharmaceutical Education and ResearchP. Ramarao - National Institute of Pharmaceutical Education and ResearchG. B. Jena - National Institute of Pharmaceutical Education and Research
- Resource Type
- Journal article
- Publication Details
- Mutation research. Genetic toxicology and environmental mutagenesis, Vol.653(1-2), pp.134-139
- Publisher
- Elsevier
- DOI
- 10.1016/j.mrgentox.2008.03.004
- PMID
- 18468946
- ISSN
- 1383-5718
- eISSN
- 1879-3592
- Number of pages
- 6
- Language
- English
- Date published
- 05/31/2008
- Academic Unit
- Cardiovascular Medicine; Fraternal Order of Eagles Diabetes Research Center; Internal Medicine
- Record Identifier
- 9984359881302771
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