Useful tips, widely used techniques, and quantifying cell metabolic behavior

David W Murhammer

doi:10.1385/1-59745-457-5:3

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Journal article

Useful tips, widely used techniques, and quantifying cell metabolic behavior

David W Murhammer

Methods in molecular biology (Clifton, N.J.), Vol.388, pp.3-22

2007

DOI: 10.1385/1-59745-457-5:3

PMID: 17951763

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Abstract

The insect cell culture/baculovirus system has three primary applications: (1) recombinant protein synthesis, (2) biopesticide synthesis, and (3) as a model system (e.g., for studying apoptosis). The fundamental techniques involved in these applications are described throughout this book. In this chapter, the most widely techniques are summarized and the reader is directed to detailed information found elsewhere in this book. Furthermore, many useful tips and the author's personal preferences that are rarely published are discussed in this chapter along with quantitative methods to characterize cell growth, baculovirus infection, and metabolism.

Animals

Baculoviridae - genetics

Cell Count

Cell Line

Cell Proliferation

Insecta - cytology

Insecta - genetics

Insecta - metabolism

Recombinant Proteins - biosynthesis

Recombinant Proteins - genetics

Details

Title: Subtitle: Useful tips, widely used techniques, and quantifying cell metabolic behavior
Creators: David W Murhammer - University of Iowa
Resource Type: Journal article
Publication Details: Methods in molecular biology (Clifton, N.J.), Vol.388, pp.3-22
DOI: 10.1385/1-59745-457-5:3
PMID: 17951763
ISSN: 1064-3745
eISSN: 1940-6029
Language: English
Date published: 2007
Academic Unit: Chemical and Biochemical Engineering
Record Identifier: 9984197249802771

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