Journal article
Using 3D-bioprinted models to study pediatric neural crest-derived tumors
International journal of bioprinting, Vol.9(4), pp.115-128
01/01/2023
DOI: 10.18063/ijb.723
PMCID: PMC10261178
PMID: 37323483
Abstract
The use of three-dimensional (3D) bioprinting has remained at the forefront of tissue engineering and has recently been employed for generating bioprinted solid tumors to be used as cancer models to test therapeutics. In pediatrics, neural crest-derived tumors are the most common type of extracranial solid tumors. There are only a few tumor-specific therapies that directly target these tumors, and the lack of new therapies remains detrimental to improving the outcomes for these patients. The absence of more efficacious therapies for pediatric solid tumors, in general, may be due to the inability of the currently employed preclinical models to recapitulate the solid tumor phenotype. In this study, we utilized 3D bioprinting to generate neural crest-derived solid tumors. The bioprinted tumors consisted of cells from established cell lines and patient-derived xenograft tumors mixed with a 6% gelatin/1% sodium alginate bioink. The viability and morphology of the bioprints were analyzed via bioluminescence and immunohisto chemistry, respectively. We compared the bioprints to traditional two-dimensional (2D) cell culture under conditions such as hypoxia and therapeutics. We successfully produced viable neural crest-derived tumors that retained the histology and immunostaining characteristics of the original parent tumors. The bioprinted tumors propagated in culture and grew in orthotopic murine models. Furthermore, compared to cells grown in traditional 2D culture, the bioprinted tumors were resistant to hypoxia and chemotherapeutics, suggesting that the bioprints exhibited a phenotype that is consistent with that seen clinically in solid tumors, thus potentially making this model superior to traditional 2D culture for preclinical investigations. Future applications of this technology entail the potential to rapidly print pediatric solid tumors for use in high -throughput drug studies, expediting the identification of novel, individualized therapies.
Details
- Title: Subtitle
- Using 3D-bioprinted models to study pediatric neural crest-derived tumors
- Creators
- Colin H. Quinn - University of Alabama at BirminghamAndee M. Beierle - University of Alabama at BirminghamJanet R. Julson - University of Alabama at BirminghamMichael E. Erwin - University of Alabama at BirminghamHasan Alrefai - University of Alabama at BirminghamHooper R. Markert - University of Alabama at BirminghamJerry E. Stewart - University of Alabama at BirminghamSara Claire Hutchins - University of Alabama at BirminghamLaura V Bownes - University of AlabamaJamie M. Aye - University of Alabama at BirminghamElizabeth Mroczek-Musulman - University of AlabamaPatricia H. Hicks - University of Alabama at BirminghamKarina J. Yoon - University of Alabama at BirminghamChristopher D. Willey - University of Alabama at BirminghamElizabeth A. Beierle - University of Alabama at Birmingham
- Resource Type
- Journal article
- Publication Details
- International journal of bioprinting, Vol.9(4), pp.115-128
- DOI
- 10.18063/ijb.723
- PMID
- 37323483
- PMCID
- PMC10261178
- NLM abbreviation
- Int J Bioprint
- ISSN
- 2424-7723
- eISSN
- 2424-8002
- Publisher
- Accscience Publishing
- Number of pages
- 14
- Grant note
- Sid Strong Foundation Elaine Roberts Foundation Open Hands Overflowing Hearts, Starr Fund-Vince Lombardi Cancer Foundation U01 CA223976; U01 CA223976-03S1 / NIH/NCI; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) 5T32GM008361; T32 CA229102; P30 CA013148 / National Cancer Institute of the National Institutes of Health; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) Hyundai Hope on Wheels
- Language
- English
- Date published
- 01/01/2023
- Academic Unit
- Stead Family Department of Pediatrics; Hematology/Oncology
- Record Identifier
- 9984701548502771
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