Journal article
Using American Type Culture Collection Cell Lines to Evaluate Interlaboratory Variables for Estrogen Receptor and Human Epidermal Growth Factor Receptor 2 Immunostaining
Archives of pathology & laboratory medicine (1976), Vol.147(2), pp.143-148
02/01/2023
DOI: 10.5858/arpa.2021-0152-CP)
Abstract
Furthermore, as the sophistication of tumor subtyping has continued to evolve, most IHC laboratories have steadily increased their menu of stains over time. [...]laboratories need to continually validate new stains in addition to performing quality assurance on their existing panels. [...]when a laboratory switches staining platforms it has to repeat a validation for its entire immunohistochemistry (IHC) menu. Often the initial, and sometimes the only, sampling of a tumor is a specimen that is handled by cytopathology. [...]this service also requires the support of IHC stains for complete evaluation of both exfoliative and fine-needle aspiration (FNA)/core biopsy cases. Preparation of a cell block is the recommended cytology specimen for ancillary testing, as it is considered the cytology equivalent of FFPE tissue,4 and some studies have shown similar performance rates between cell blocks and surgical core biopsy samples.4-7 In summary, the cell block is especially valuable in providing a concentration of cells in a relatively small area of the slide, functions as a histologic correlate of the cytology material, and allows for additional sections to be produced for ancillary testing to further classify malignant cases.8-10 Since cell blocks are processed in a manner akin to FFPE tissue blocks from surgical pathology, they generally do not undergo additional validation.8,11 It is important to recognize that different laboratories introduce added preanalytic variability into their methods by using different preservatives for cytology specimen collection and substantial interlaboratory variation in procedures for generating cell blocks.
Details
- Title: Subtitle
- Using American Type Culture Collection Cell Lines to Evaluate Interlaboratory Variables for Estrogen Receptor and Human Epidermal Growth Factor Receptor 2 Immunostaining
- Creators
- Benjamin WittWenhua ZhouAbiy AmbayeAndrew BellizziChristine BoothKaitlin SundlingLananh NguyenDonna RussellMalcolm SchinstinePaul StaatsJean ThomsenMegan TroxellRhona SouersJames DvorakXiaoqi LinDaniel Kurtycz
- Resource Type
- Journal article
- Publication Details
- Archives of pathology & laboratory medicine (1976), Vol.147(2), pp.143-148
- Publisher
- College of American Pathologists
- DOI
- 10.5858/arpa.2021-0152-CP)
- ISSN
- 0003-9985
- eISSN
- 1543-2165
- Language
- English
- Date published
- 02/01/2023
- Academic Unit
- Pathology
- Record Identifier
- 9984363656602771
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