Journal article
Using cyclic peptide mixtures as probes for metal ion host-guest interactions
Letters in peptide science : LIPS, Vol.3(1), pp.17-24
03/01/1996
DOI: 10.1007/BF00131081
Abstract
Summary
Mixtures of cyclic peptides, formed by head-to-tail cyclizations of side-chain resin-bound linear sequences, have been prepared using solid-phase synthesis. Fast atom bombardment mass spectrometry of cyclic peptides with various metal ions can reveal preferred modes of host-guest patterns, albeit in a nonquantitative manner. This approach could prove useful for more rapid screening of potential peptide ionophores. A cyclic heptapeptide with a dipeptide tail proved to be a particularly effective host for a Ca2+ ion; in a small three-component mixture, cyclo[Gly-Asp-D-Pro-Xxx-Asp-D-Pro-Asp(Aca-Phe-NH2)], binding to Ca2+ varied from Xxx=N-MeAla>Gly≈Sar. In a 15-component mixture, cyclo[Pro-Xxx-Asn-Pro-Xxx-Asn] where Xxx=Ala, Glu, Leu, Lys or Phe, there were no significant differences with respect to binding to metal ions. We believe this to be the first reported use of cyclic peptide libraries for screening metal ions to discern host-guest relationships.
Details
- Title: Subtitle
- Using cyclic peptide mixtures as probes for metal ion host-guest interactions
- Creators
- Jian J. Chen - University of LouisvilleLynn M. Teesch - University of IowaArno F. Spatola - University of Louisville
- Resource Type
- Journal article
- Publication Details
- Letters in peptide science : LIPS, Vol.3(1), pp.17-24
- DOI
- 10.1007/BF00131081
- ISSN
- 0929-5666
- eISSN
- 1573-496X
- Language
- English
- Date published
- 03/01/1996
- Academic Unit
- Medicine Administration; Core Research Facilities
- Record Identifier
- 9984622054702771
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