Journal article
Using proteomic analysis of the human amniotic fluid to identify histologic chorioamnionitis
Obstetrics and gynecology (New York. 1953), Vol.111(2), pp.403-412
02/01/2008
DOI: 10.1097/AOG.0b013e31816102aa
PMID: 18238979
Abstract
OBJECTIVE: To estimate the relationship between histologic chorioamnionitis and four amniotic fluid proteomic biomarkers characteristic of inflammation (defensins 2 and 1, calgranulins C and A).
METHODS: One hundred fifty-eight women with singleton pregnancies had a clinically indicated amniocentesis to rule out inflammation and infection in the context of preterm labor or preterm premature rupture of membranes. A proteomic fingerprint (Mass Restricted score) was generated from amniotic fluid using surface-enhanced laser desorption ionization time-of-flight mass spectrometry. The Mass Restricted score ranges from 0 to 4 (none to all four biomarkers present) in direct relationship with severity of intra-amniotic inflammation. Presence or absence of biomarkers was analyzed in relationship to placental pathology. Criteria for severity of histologic chorioamnionitis were 3 stages and 4 grades of inflammation of the amnion, choriodecidua and chorionic plate.
RESULTS: The prevalence of histologic chorioamnionitis was 64% (stage I 12 /0, stage II 16%, and stage III 37%). The Mass Restricted score significantly correlated with stages of histologic chorioamnionitis (r=0.539, P<.001), grades of choriodeciduitis (r=0.465, P<.001), and amnionitis (r=0.536, P<.001). African-American women were overrepresented in the group with severe inflammation (Mass Restricted score 3-4, P=.022). Of the four biomarkers of the Mass Restricted score, calgranulin C had the strongest relationship with presence of stage III chorioamnionitis, independent of race, amniocentesis-to-delivery interval, and gestational age.
CONCLUSION: Proteomic analysis of amniotic fluid provides an opportunity for early recognition of histologic chorioamnionitis. This methodology may in the future identify candidates for antenatal therapeutic interventions.
Details
- Title: Subtitle
- Using proteomic analysis of the human amniotic fluid to identify histologic chorioamnionitis
- Creators
- Irina A. Buhimschi - Yale UniversityEduardo Zambrano - Yale UniversityChristian M. Pettker - Yale UniversityMert Ozan Bahtiyar - Yale UniversityMichael Paidas - Yale UniversityVictor A. Rosenberg - Yale UniversityStephen Thung - Yale UniversityCarolyn M. Salafia - Yale UniversityCatalin S. Buhimschi - Yale University
- Resource Type
- Journal article
- Publication Details
- Obstetrics and gynecology (New York. 1953), Vol.111(2), pp.403-412
- DOI
- 10.1097/AOG.0b013e31816102aa
- PMID
- 18238979
- NLM abbreviation
- Obstet Gynecol
- ISSN
- 0029-7844
- eISSN
- 1873-233X
- Publisher
- Lippincott Williams & Wilkins
- Number of pages
- 10
- Grant note
- R01HD047321 / EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH &HUMAN DEVELOPMENT; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH Eunice Kennedy Shriver National Institute of Child Health & Human Development (NICHD)
- Language
- English
- Date published
- 02/01/2008
- Academic Unit
- Obstetrics and Gynecology
- Record Identifier
- 9985244563702771
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