Journal article
Utility of Flow Cytometry and Fluorescence In Situ Hybridization in Follow-up Monitoring of Plasma Cell Myeloma
American journal of clinical pathology, Vol.156(2), pp.198-204
01/13/2021
DOI: 10.1093/ajcp/aqaa224
PMID: 33437993
Abstract
Abstract
Objectives
We sought to investigate the clinical utility of flow cytometry (FC) and fluorescence in situ hybridization (FISH) in the workup of myeloma.
Methods
We retrospectively reviewed the reports of bone marrow biopsies received for myeloma evaluation between October 2015 and January 2019.
Results
A total of 1,708 biopsy specimens from 469 myeloma patients (mean age, 64.5 years [SD, 9.3]; female, 41.4%) were reviewed. Both FC and FISH had comparable detection rates at the time of initial diagnosis (97.6% vs 98.8%) and for follow-up cases (28.6% vs 28.2%). FC and FISH results were concordant in 98.8% of the initial diagnosis cases and 89.6% of the follow-up cases. The FISH-positive (FISH+)/FC-negative (FC−) discordance and FISH−/FC+ discordance occurred among 81 (5.0%) and 87 (5.4%) follow-up cases. In comparison with all concordant cases, FISH+/FC− discordant cases were more likely to have received treatment with daratumumab (P < .05).
Conclusions
Plasma cell–enriched FISH and FC have comparable abnormal plasma cell detection rates, and approximately 10% of the follow-up cases have discordant FISH and FC results in which residual disease is detected by only one of these modalities. FISH testing should be considered for cases with negative FC, especially in patients who have received treatment with daratumumab or in cases in which there is concern about specimen adequacy.
Details
- Title: Subtitle
- Utility of Flow Cytometry and Fluorescence In Situ Hybridization in Follow-up Monitoring of Plasma Cell Myeloma
- Creators
- Saurav Chopra - Department of Pathology.Timothy Dunham - University of Iowa Hospitals and ClinicsSergei I Syrbu - Department of Pathology.Nitin J Karandikar - Department of Pathology.Benjamin W Darbro - University of Iowa Hospitals and ClinicsCarol J Holman - Department of Pathology.
- Resource Type
- Journal article
- Publication Details
- American journal of clinical pathology, Vol.156(2), pp.198-204
- Publisher
- Oxford University Press
- DOI
- 10.1093/ajcp/aqaa224
- PMID
- 33437993
- ISSN
- 0002-9173
- eISSN
- 1943-7722
- Grant note
- name: Department of Pathology; name: Stead Family Department of Pediatrics; name: University of Iowa Hospital and Clinics
- Language
- English
- Date published
- 01/13/2021
- Academic Unit
- Stead Family Department of Pediatrics; Pathology; Medical Genetics and Genomics
- Record Identifier
- 9984186536802771
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