Utilization of the mating scaffold protein in the evolution of a new signal transduction pathway for biofilm development

Song Yi; Nidhi Sahni; Karla J Daniels; Kevin L Lu; Guanghua Huang; Adam M Garnaas; Claude Pujol; Thyagarajan Srikantha; David R Soll

doi:10.1128/mBio.00237-10

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Utilization of the mating scaffold protein in the evolution of a new signal transduction pathway for biofilm development

Journal article

Open access

Peer reviewed

Utilization of the mating scaffold protein in the evolution of a new signal transduction pathway for biofilm development

Song Yi, Nidhi Sahni, Karla J Daniels, Kevin L Lu, Guanghua Huang, Adam M Garnaas, Claude Pujol, Thyagarajan Srikantha and David R Soll

mBio, Vol.2(1), pp.e00237-e00210

01/11/2011

DOI: 10.1128/mBio.00237-10

PMCID: PMC3018282

PMID: 21221248

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Abstract

Among the hemiascomycetes, only Candida albicans must switch from the white phenotype to the opaque phenotype to mate. In the recent evolution of this transition, mating-incompetent white cells acquired a unique response to mating pheromone, resulting in the formation of a white cell biofilm that facilitates mating. All of the upstream components of the white cell response pathway so far analyzed have been shown to be derived from the ancestral pathway involved in mating, except for the mitogen-activated protein (MAP) kinase scaffold protein, which had not been identified. Here, through binding and mutational studies, it is demonstrated that in both the opaque and the white cell pheromone responses, Cst5 is the scaffold protein, supporting the evolutionary scenario proposed. Although Cst5 plays the same role in tethering the MAP kinases as Ste5 does in Saccharomyces cerevisiae, Cst5 is approximately one-third the size and has only one rather than four phosphorylation sites involved in activation and cytoplasmic relocalization.

Gene Expression Regulation, Fungal

Signal Transduction

Biofilms - growth & development

Nuclear Matrix-Associated Proteins - metabolism

Fungal Proteins - genetics

Pheromones - metabolism

Candida albicans - growth & development

Biological Evolution

Candida albicans - genetics

Nuclear Matrix-Associated Proteins - genetics

Protein Binding

Candida albicans - physiology

Genes, Mating Type, Fungal

Fungal Proteins - metabolism

Details

Title: Subtitle: Utilization of the mating scaffold protein in the evolution of a new signal transduction pathway for biofilm development
Creators: Song Yi - The Developmental Studies Hybridoma Bank-Microbe, Department of Biology, the University of Iowa, Iowa City, Iowa, USA
Nidhi Sahni
Karla J Daniels
Kevin L Lu
Guanghua Huang
Adam M Garnaas
Claude Pujol
Thyagarajan Srikantha
David R Soll
Resource Type: Journal article
Publication Details: mBio, Vol.2(1), pp.e00237-e00210
DOI: 10.1128/mBio.00237-10
PMID: 21221248
PMCID: PMC3018282
NLM abbreviation: mBio
ISSN: 2150-7511
eISSN: 2150-7511
Publisher: United States
Language: English
Date published: 01/11/2011
Academic Unit: Biology; Internal Medicine
Record Identifier: 9983991977002771

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