Utilizing pyrosequencing and quantitative PCR to characterize fungal populations among house dust samples

Matthew W Nonnenmann; Gloria Coronado; Beti Thompson; William C Griffith; John Delton Hanson; Stephen Vesper; Elaine M Faustman

doi:10.1039/c2em30229b

Journal article

Utilizing pyrosequencing and quantitative PCR to characterize fungal populations among house dust samples

Matthew W Nonnenmann, Gloria Coronado, Beti Thompson, William C Griffith, John Delton Hanson, Stephen Vesper and Elaine M Faustman

Journal of environmental monitoring, Vol.14(8), pp.2038-243

08/2012

DOI: 10.1039/c2em30229b

PMCID: PMC4079115

PMID: 22767010

Files and links (1)

url

https://www.ncbi.nlm.nih.gov/pmc/articles/4079115View

Open Access

Abstract

Molecular techniques are an alternative to culturing and counting methods in quantifying indoor fungal contamination. Pyrosequencing offers the possibility of identifying unexpected indoor fungi. In this study, 50 house dust samples were collected from homes in the Yakima Valley, WA. Each sample was analyzed by quantitative PCR (QPCR) for 36 common fungi and by fungal tag-encoded flexible (FLX) amplicon pyrosequencing (fTEFAP) for these and additional fungi. Only 24 of the samples yielded amplified results using fTEFAP but QPCR successfully amplified all 50 samples. Over 450 fungal species were detected by fTEFAP but most were rare. Twenty-two fungi were found by fTEFAP to occur with at least an average of ≥0.5% relative occurrence. Many of these fungi seem to be associated with plants, soil or human skin. Combining fTEFAP and QPCR can enhance studies of fungal contamination in homes.

Air Microbiology

Air Pollution, Indoor - analysis

Dust - analysis

Fungi - classification

Fungi - genetics

Housing

Humans

Polymerase Chain Reaction

Washington

Details

Title: Subtitle: Utilizing pyrosequencing and quantitative PCR to characterize fungal populations among house dust samples
Creators: Matthew W Nonnenmann - Department of Occupational and Environmental Health, University of Iowa, Iowa City, Iowa 52242, USA. matthew-nonnenmann@uiowa.edu
Gloria Coronado
Beti Thompson
William C Griffith
John Delton Hanson
Stephen Vesper
Elaine M Faustman
Resource Type: Journal article
Publication Details: Journal of environmental monitoring, Vol.14(8), pp.2038-243
DOI: 10.1039/c2em30229b
PMID: 22767010
PMCID: PMC4079115
NLM abbreviation: J Environ Monit
ISSN: 1464-0325
eISSN: 1464-0333
Grant note: HHSN267200700023C. / PHS HHS P01 ES009601 / NIEHS NIH HHS HHSN267200700023C / NICHD NIH HHS ES-09601 / NIEHS NIH HHS
Language: English
Date published: 08/2012
Academic Unit: Occupational and Environmental Health; Internal Medicine
Record Identifier: 9984214784102771

Metrics

23 Record Views

24 Times Cited - Web of Science